Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca

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dc.contributor.authorFernández-Aparicio, Mónicaen
dc.contributor.authorRubiales, Diegoen
dc.contributor.authorBandaranayake, Pradeepa C.G.en
dc.contributor.authorYoder, John I.en
dc.contributor.authorWestwood, James H.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-09-10T15:18:11Zen
dc.date.available2019-09-10T15:18:11Zen
dc.date.issued2011en
dc.description.abstractBackground: Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project) will fuel the use of genomic tools for studies on parasitic plants. A reliable system for holoparasite transformation is needed to realize the full value of this resource for reverse genetics and functional genomics studies. Results: Here we demonstrate that transformation of Phelipanche aegyptiaca is achieved by infection of 3 monthold in vitro grown P. aegyptiaca calli with Agrobacterium rhizogenes harboring the yellow fluorescent protein (YFP). Four months later, YFP-positive regenerated calli were inoculated onto tomato plants growing in a minirhizotron system. Eight days after inoculation, transgenic parasite tissue formed lateral haustoria that penetrated the host and could be visualized under UV illumination through intact host root tissue. YFP-positive shoot buds were observed one month after inoculation. Conclusions: This work constitutes a breakthrough in holoparasitic plant research methods. The method described here is a robust system for transformation and regeneration of a holoparasitic plant and will facilitate research on unique parasitic plant capabilities such as host plant recognition, haustorial formation, penetration and vascular connection.en
dc.description.sponsorshipThis research is supported by International Outgoing European Marie Curie postdoctoral fellowship (PIOF-GA-2009-252538) to M Fernández-Aparicio; and projects P07-AGR-02883 and AGL2008-01239/AGR co-financed by FEDER funds to D Rubiales, NSF Plant Genome award DBI-0701748 to JH Westwood and JI Yoder, and U.S. Department of Agriculture (Hatch Project no. 135798) to JH Westwood.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1186/1746-4811-7-36en
dc.identifier.issue36en
dc.identifier.urihttp://hdl.handle.net/10919/93521en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherBMCen
dc.rightsCreative Commons Attribution 3.0 United Statesen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjectholoparasitic plantsen
dc.subjectgene transformationen
dc.subjecthaustoriumen
dc.subjectPhelipancheen
dc.subjectOrobancheen
dc.titleTransformation and regeneration of the holoparasitic plant Phelipanche aegyptiacaen
dc.title.serialPlant Methodsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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