Single-Cell Analysis Reveals that Chronic Silver Nanoparticle Exposure Induces Cell Division Defects in Human Epithelial Cells

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dc.contributor.authorGarcia, Ellen B.en
dc.contributor.authorAlms, Cynthiaen
dc.contributor.authorHinman, Albert W.en
dc.contributor.authorKelly, Conoren
dc.contributor.authorSmith, Adamen
dc.contributor.authorVance, Marinaen
dc.contributor.authorLoncarek, Jadrankaen
dc.contributor.authorMarr, Linsey C.en
dc.contributor.authorCimini, Danielaen
dc.contributor.departmentCivil and Environmental Engineeringen
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-06-14T12:56:28Zen
dc.date.available2019-06-14T12:56:28Zen
dc.date.issued2019-06-11en
dc.date.updated2019-06-14T07:32:29Zen
dc.description.abstractMultiple organizations have urged a paradigm shift from traditional, whole animal, chemical safety testing to alternative methods. Although these forward-looking methods exist for risk assessment and predication, animal testing is still the preferred method and will remain so until more robust cellular and computational methods are established. To meet this need, we aimed to develop a new, cell division-focused approach based on the idea that defective cell division may be a better predictor of risk than traditional measurements. To develop such an approach, we investigated the toxicity of silver nanoparticles (AgNPs) on human epithelial cells. AgNPs are the type of nanoparticle most widely employed in consumer and medical products, yet toxicity reports are still confounding. Cells were exposed to a range of AgNP doses for both short- and-long term exposure times. The analysis of treated cell populations identified an effect on cell division and the emergence of abnormal nuclear morphologies, including micronuclei and binucleated cells. Overall, our results indicate that AgNPs impair cell division, not only further confirming toxicity to human cells, but also highlighting the propagation of adverse phenotypes within the cell population. Furthermore, this work illustrates that cell division-based analysis will be an important addition to future toxicology studies.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGarcia, E.B.; Alms, C.; Hinman, A.W.; Kelly, C.; Smith, A.; Vance, M.; Loncarek, J.; Marr, L.C.; Cimini, D. Single-Cell Analysis Reveals that Chronic Silver Nanoparticle Exposure Induces Cell Division Defects in Human Epithelial Cells. Int. J. Environ. Res. Public Health 2019, 16, 2061.en
dc.identifier.doihttps://doi.org/10.3390/ijerph16112061en
dc.identifier.urihttp://hdl.handle.net/10919/90171en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectsilver nanoparticlesen
dc.subjectmitosisen
dc.subjectnanotoxicologyen
dc.subjectmicronucleusen
dc.subjecttetraploidyen
dc.titleSingle-Cell Analysis Reveals that Chronic Silver Nanoparticle Exposure Induces Cell Division Defects in Human Epithelial Cellsen
dc.title.serialInternational Journal of Environmental Research and Public Healthen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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