A feminizing switch in a hemimetabolous insect

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dc.contributor.authorZhuo, Ji-Chongen
dc.contributor.authorZhang, Hou-Hongen
dc.contributor.authorHu, Qing-Lingen
dc.contributor.authorZhang, Jin-Lien
dc.contributor.authorLu, Jia-Baoen
dc.contributor.authorLi, Han-Jingen
dc.contributor.authorXie, Yu-Chengen
dc.contributor.authorWang, Wei-Weien
dc.contributor.authorZhang, Yanen
dc.contributor.authorWang, Hai-Qiangen
dc.contributor.authorHuang, Hai-Jianen
dc.contributor.authorLu, Gangen
dc.contributor.authorChen, Jian-Pingen
dc.contributor.authorLi, Jun-Minen
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorZhang, Chuan-Xien
dc.date.accessioned2022-01-18T02:05:41Zen
dc.date.available2022-01-18T02:05:41Zen
dc.date.issued2021-11-01en
dc.date.updated2022-01-18T02:05:36Zen
dc.description.abstractThe mechanism of sex determination remains poorly understood in hemimetabolous insects. Here, in the brown planthopper (BPH), Nilaparvata lugens, a hemipteran rice pest, we identified a feminizing switch or a female determiner (Nlfmd) that encodes a serine/arginine-rich protein. Knockdown of Nlfmd in female nymphs resulted in masculinization of both the somatic morphology and doublesex splicing. The female-specific isoform of Nlfmd, Nlfmd-F, is maternally deposited and zygotically transcribed. Depletion of Nlfmd by maternal RNAi or CRISPR-Cas9 resulted in female-specific embryonic lethality. Knockdown of an hnRNP40 family gene named female determiner 2 (Nlfmd2) also conferred masculinization. In vitro experiments showed that an Nlfmd2 isoform, NlFMD2340, bound the RAAGAA repeat motif in the Nldsx pre-mRNA and formed a protein complex with NlFMD-F to modulate Nldsx splicing, suggesting that NlFMD2 may function as an RNA binding partner of the feminizing switch NlFMD. Our results provide novel insights into the diverse mechanisms of insect sex determination.en
dc.description.versionPublished versionen
dc.format.extent13 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN eabf9237 (Article number)en
dc.identifier.doihttps://doi.org/10.1126/sciadv.abf9237en
dc.identifier.eissn2375-2548en
dc.identifier.issn2375-2548en
dc.identifier.issue48en
dc.identifier.orcidTu, Zhijian [0000-0003-4227-3819]en
dc.identifier.pmid34826246en
dc.identifier.urihttp://hdl.handle.net/10919/107710en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherAAASen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000722925100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectSEX DETERMINATIONen
dc.subjectCERATITIS-CAPITATAen
dc.subjectBROWN PLANTHOPPERen
dc.subjectTRANSFORMER GENEen
dc.subjectRNAen
dc.subjectDROSOPHILAen
dc.subjectDOUBLESEXen
dc.subjectPATHWAYSen
dc.subjectSEQUENCEen
dc.subjectTRA-2en
dc.titleA feminizing switch in a hemimetabolous insecten
dc.title.serialScience Advancesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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