Genotyping-by-sequencing analysis of Orobanche crenata populations in Algeria reveals genetic differentiation

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dc.contributor.authorBendaoud, Farahen
dc.contributor.authorKim, Gunjuneen
dc.contributor.authorLarose, Haileyen
dc.contributor.authorWestwood, James H.en
dc.contributor.authorZermane, Nadjiaen
dc.contributor.authorHaak, David C.en
dc.coverage.countryAlgeriaen
dc.date.accessioned2022-07-18T13:51:25Zen
dc.date.available2022-07-18T13:51:25Zen
dc.date.issued2022-03en
dc.description.abstractCrenate broomrape (Orobanche crenata Forsk.) is a serious long-standing parasitic weed problem in Algeria, mainly affecting legumes but also vegetable crops. Unresolved questions for parasitic weeds revolve around the extent to which these plants undergo local adaptation, especially with respect to host specialization, which would be expected to be a strong selective factor for obligate parasitic plants. In the present study, the genotyping-by-sequencing (GBS) approach was used to analyze genetic diversity and population structure of 10 Northern Algerian O. crenata populations with different geographical origins and host species (faba bean, pea, chickpea, carrot, and tomato). In total, 8004 high-quality single-nucleotide polymorphisms (5% missingness) were obtained and used across the study. Genetic diversity and relationships of 95 individuals from 10 populations were studied using model-based ancestry analysis, principal components analysis, discriminant analysis of principal components, and phylogeny approaches. The genetic differentiation (F-ST) between pairs of populations was lower between adjacent populations and higher between geographically separated ones, but no support was found for isolation by distance. Further analyses identified four genetic clusters and revealed evidence of structuring among populations and, although confounded with location, among hosts. In the clearest example, O. crenata growing on pea had a SNP profile that was distinct from other host/location combinations. These results illustrate the importance and potential of GBS to reveal the dynamics of parasitic weed dispersal and population structure.en
dc.description.notesNational Institute of Food and Agriculture, Grant/Award Number: VA-160055 and VA-160111; National Science Foundation, Grant/Award Number: IOS-1238057en
dc.description.sponsorshipNational Institute of Food and Agriculture [VA-160055, VA-160111]; National Science Foundation [IOS-1238057]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/ece3.8750en
dc.identifier.issn2045-7758en
dc.identifier.issue3en
dc.identifier.othere8750en
dc.identifier.pmid35356582en
dc.identifier.urihttp://hdl.handle.net/10919/111274en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherWileyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAlgeriaen
dc.subjectGBSen
dc.subjectgenetic diversityen
dc.subjectgenotyping by sequencingen
dc.subjectOrobanche crenataen
dc.subjectpopulation structureen
dc.titleGenotyping-by-sequencing analysis of Orobanche crenata populations in Algeria reveals genetic differentiationen
dc.title.serialEcology and Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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