Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system

Simple item page
dc.contributor.authorMao, Chunhongen
dc.contributor.authorEvans, Cliveen
dc.contributor.authorJensen, Roderick V.en
dc.contributor.authorSobral, Brunoen
dc.date.accessioned2012-08-24T11:55:52Zen
dc.date.available2012-08-24T11:55:52Zen
dc.date.issued2008-05-02en
dc.date.updated2012-08-24T11:55:52Zen
dc.description.abstractBackground Sinorhizobium meliloti is an agriculturally important model symbiont. There is an ongoing need to update and improve its genome annotation. In this study, we used a high-throughput pyrosequencing approach to sequence the transcriptome of S. meliloti, and search for new bacterial genes missed in the previous genome annotation. This is the first report of sequencing a bacterial transcriptome using the pyrosequencing technology. Results Our pilot sequencing run generated 19,005 reads with an average length of 136 nucleotides per read. From these data, we identified 20 new genes. These new gene transcripts were confirmed by RT-PCR and their possible functions were analyzed. Conclusion Our results indicate that high-throughput sequence analysis of bacterial transcriptomes is feasible and next-generation sequencing technologies will greatly facilitate the discovery of new genes and improve genome annotation.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Microbiology. 2008 May 02;8(1):72en
dc.identifier.doihttps://doi.org/10.1186/1471-2180-8-72en
dc.identifier.urihttp://hdl.handle.net/10919/18889en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderChunhong Mao et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIdentification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX systemen
dc.title.serialBMC Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 4 of 4
Thumbnail Image
Name:
1471-2180-8-72.pdf
Size:
313.88 KB
Format:
Adobe Portable Document Format
Download
Name:
1471-2180-8-72-S1.XLS
Size:
154 KB
Format:
Microsoft Excel
Download
Name:
1471-2180-8-72-S2.XLS
Size:
23 KB
Format:
Microsoft Excel
Download
Name:
1471-2180-8-72-S3.XLS
Size:
19 KB
Format:
Microsoft Excel
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, BioMed Central and SpringerOpen
Scholarly Works, Fralin Life Sciences Institute