Prostaglandins Modulate the Uterine Response to Infectious Bacteria in Postpartum and Estrous Cyclic Ewes

Simple item page
dc.contributor.authorSeals, Richarden
dc.contributor.committeecochairKnight, James W.en
dc.contributor.committeecochairLewis, Gregory S.en
dc.contributor.committeememberSponenberg, D. Phillipen
dc.contributor.committeememberElgert, Klaus D.en
dc.contributor.committeememberHohenboken, William D.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2014-03-14T20:11:00Zen
dc.date.adate2000-04-28en
dc.date.available2014-03-14T20:11:00Zen
dc.date.issued2000-04-21en
dc.date.rdate2001-04-28en
dc.date.sdate2000-04-27en
dc.description.abstractThe uterine immune system is down regulated when exposed to P4. Five experiments were conducted to determine the in vivo and in vitro role(s) of P4 and prostaglandins in immune function(s) associated with uterine infections. In Exp. 1, postpartum ewes (d 0 = parturition) were supplemented with either OIL or P4 (beginning on d 10) or were SHAM or OVEX. Vena caval blood and lymphocytes were collected on d 14, and 16 to 19. All ewes received intrauterine inoculations of bacteria on d 15 and uteri were collected on d 20. Ewes receiving P4 developed infections. Lymphocytes were incubated with mitogens, PGE2, indomethacin (INDO; a PG synthesis inhibitor) or both in a 3 x 2 x 2 arrangement. Concanavalin A-stimulated blastogenesis in P4-OVEX ewes and PGE2 and PGE2+INDO treated lymphocytes was inhibited (P < .05). Cyclic ewes in their follicular or luteal phase received either intrauterine inoculations of saline or bacteria, vena caval blood was collected for 3 d, and uteri were collected. Lymphocytes were incubated with mitogens, PGE2 (Exp. 2), PGF2alpha (Exp. 3) and(or) INDO in a 3 x 2 x 2 arrangement. Only luteal phase ewes that received bacteria developed infections. In Exp. 2, Con A- and LPS-stimulated blastogenesis was greater for luteal than for follicular phase ewes. T lymphocyte proliferation was inhibited in ewes inoculated with bacteria. T lymphocyte proliferation tended to be higher (P = .09) when incubated with INDO. In Exp. 3, T lymphocyte proliferation in response to PGF2alpha was greater for follicular than for luteal phase ewes. Neutrophils were lower in ewes inoculted with bacteria. In Exp. 4 and 5, uteri of luteal-phase (d 6) ewes were inoculated with bacteria. Ewes received either 15 mg of Lutalyse or saline on d 9, and uteri were collected on d 11. Lutalyse reduced P4, tended to decrease neutrophils, allowed ewes to clear infections, and had no effect on blastogenesis. Methods for modulating uterine prostaglandins seem to reduce susceptibility to uterine infections.en
dc.description.degreePh. D.en
dc.identifier.otheretd-04272000-16590044en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04272000-16590044/en
dc.identifier.urihttp://hdl.handle.net/10919/27345en
dc.publisherVirginia Techen
dc.relation.haspartrseals-etd.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectuterine infectionsen
dc.subjectprostaglandinsen
dc.subjectsheepen
dc.subjectcattleen
dc.subjectlymphocytesen
dc.titleProstaglandins Modulate the Uterine Response to Infectious Bacteria in Postpartum and Estrous Cyclic Ewesen
dc.typeDissertationen
thesis.degree.disciplineAnimal and Poultry Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
rseals-etd.pdf
Size:
836.66 KB
Format:
Adobe Portable Document Format
Download

Collections

Doctoral Dissertations