Leaf Endophyte Community Composition and Network Structures Differ Between Tolerant and Susceptible English Boxwood

dc.contributor.authorKong, Pingen
dc.contributor.authorLi, Xiaopingen
dc.contributor.authorSharifi, Melissaen
dc.contributor.authorBordas, Adriaen
dc.contributor.authorHong, Chuanxueen
dc.date.accessioned2025-01-15T18:46:21Zen
dc.date.available2025-01-15T18:46:21Zen
dc.date.issued2023-09en
dc.description.abstractDifferential tolerance of English boxwood to boxwood blight has been linked to the ratio of culturable bacterial and fungal dominance in the leaf tissue of representative samples. To further understand how the whole endophyte communities may involve the tolerance of large samples, we extracted DNA from healthy leaf tissue of previously identified 28 tolerant (T), 41 moderately tolerant (M), and 21 susceptible (S) English boxwood plants, then sequenced associated bacterial and fungal amplicons using the Nanopore MinION platform. The endophyte community did not differ in diversity among the T, M, and S plants but differed in the abundance of bacteria and fungi, particularly between T and S samples. The bacterial genera Brevundimonas and Ammonifex had higher relative abundance in the T and M communities than in the S community, in which the fungal genera Botrytis and Thermothelomyces and family Chaetomiaceae were more dominant. The same results were obtained when mother and daughter samples in the T community were compared with controls in the S community, suggesting bacteria as a work force in the T community. Cooccurrence network analyses revealed that the T network had more fungal hubs but was less complex, with more positive connections than the S network, suggesting that the T community was supported by a healthier network. The resistance of English boxwood to blight is likely attributed to bacteria dominance and a synergic community network. This study is foundational to constructing synthetic communities and using whole communities of tolerant plants through vegetative propagation for microbemodulated immunity.en
dc.description.versionAccepted versionen
dc.format.extentPages 160-171en
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1094/PBIOMES-02-23-0009-FIen
dc.identifier.eissn2471-2906en
dc.identifier.issn2471-2906en
dc.identifier.issue2en
dc.identifier.orcidHong, Chuanxue [0000-0001-7389-5157]en
dc.identifier.orcidLi, Xiaoping [0000-0003-1288-6438]en
dc.identifier.urihttps://hdl.handle.net/10919/124206en
dc.identifier.volume7en
dc.language.isoenen
dc.language.isoenen
dc.publisherAmerican Phytopathological Societyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectendophytesen
dc.subjectmetagenomicsen
dc.subjectmicrobiomeen
dc.subjectplant pathologyen
dc.titleLeaf Endophyte Community Composition and Network Structures Differ Between Tolerant and Susceptible English Boxwooden
dc.title.serialPhytobiomes Journalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciencesen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/Hampton Roads ARECen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/Virginia Cooperative Extensionen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/Virginia Cooperative Extension/Northern Districten

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