Isolation and characterization of plasmids from human and environmental isolates of mycobacteria

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dc.contributor.authorMeissner, Paul Scotten
dc.contributor.committeechairFalkinham, Joseph O. IIIen
dc.contributor.committeememberBates, Robert C.en
dc.contributor.committeememberTolin, Sue A.en
dc.contributor.committeememberStout, Ernest R.en
dc.contributor.committeememberJohnson, J.L.en
dc.contributor.departmentGeneticsen
dc.contributor.departmentGeneticsen
dc.date.accessioned2019-01-31T18:06:53Zen
dc.date.available2019-01-31T18:06:53Zen
dc.date.issued1984en
dc.description.abstractHuman clinical (n=131) and environmental (n=226) isolates of the Mycobacterium avium-intracellulare and M. scrofulaceum (MAIS) complex were screened for plasmids in an effort to increase knowledge about the genetics and epidemiology of these pathogenic bacteria. Approximately 50% of the clinical MAIS isolates from New York, Maryland, Virginia, South Carolina, and Georgia contained one or more plasmids. On the basis of plasmid content, aerosol MAIS isolates more closely resembled human MAIS isolates than did MAIS isolates from the other environmental sources examined (dust, soil, sediment, and water). Plasmid profiles were remarkably heterogenous, and isolates with identical profiles were rarely encountered. However, a 115 megadalton (Md) plasmid was detected in 15 mercury resistant human and environmental isolates. In one of these isolates (M. scrofulaceum W262) the presence of the 115 Md plasmid was shown to correlate with the presence of an NAD(P)H dependent mercuric reductase. Plasmids with molecular weights of 8.8, 11.2, 14.2, 16.9, 17.9, and 18.3 Md were also common among both human and environmental isolates. On the basis of molecular weight, 36 distinct plasmids were detected; their sizes ranged from 7 to 230 Md. It was concluded that human and environmental MAIS isolates share a number of plasmids with identical molecular weights and that plasmids can serve as useful entities in genetic and epidemiologic studies of this group of extremely slow-growing, poorly understood human and animal pathogens.en
dc.description.degreePh. D.en
dc.format.extentvii, 88 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/87173en
dc.language.isoen_USen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 11823242en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1984.M442en
dc.subject.lcshBacterial geneticsen
dc.subject.lcshMycobacteria -- Experimentsen
dc.subject.lcshPlasmids -- Experimentsen
dc.titleIsolation and characterization of plasmids from human and environmental isolates of mycobacteriaen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineGeneticsen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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