Characterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1

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dc.contributor.authorLatimer, Matthew T.en
dc.contributor.committeechairFerry, James G.en
dc.contributor.committeememberDean, Dennis R.en
dc.contributor.committeememberJohnson, John L.en
dc.contributor.committeememberFalkinham, Joseph O. IIIen
dc.contributor.committeememberWilkins, Tracy D.en
dc.contributor.departmentBiochemistry and Anaerobic Microbiologyen
dc.date.accessioned2014-03-14T21:21:49Zen
dc.date.adate2005-10-20en
dc.date.available2014-03-14T21:21:49Zen
dc.date.issued1993-10-05en
dc.date.rdate2005-10-20en
dc.date.sdate2005-10-20en
dc.description.abstractThe genes encoding the acetate kinase and phosphotransacetylase enzymes from <i>Methanosarcina thermophila</i> were isolated from a genomic library on a fifteen kilobase fragment The genes are located adjacent to one another, with the phosphotransacetylase gene (<i>pta</i>) directly upstream of the acetate kinase gene (<i>ack</i>). The two genes were sequenced, along with a third Open Reading Frame (designated <i>orfY</i>). The <i>orfY</i> gene appears to encode a novel protein whose physiological function has yet to be determined.en
dc.description.degreePh. D.en
dc.format.extentx, 146 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10202005-102824en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10202005-102824/en
dc.identifier.urihttp://hdl.handle.net/10919/40034en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1993.L374.pdfen
dc.relation.isformatofOCLC# 29700006en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1993.L374en
dc.subject.lcshAcetates -- Microbiologyen
dc.subject.lcshFlavoproteinsen
dc.subject.lcshMethanosarcina thermophilaen
dc.titleCharacterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1en
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Anaerobic Microbiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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