Longitudinal Shedding Patterns and Characterization of Antibiotic Resistant E. coli in Pastured Goats Using a Cohort Study

dc.contributor.authorNdegwa, Euniceen
dc.contributor.authorAlmehmadi, Haninen
dc.contributor.authorKim, Chyeren
dc.contributor.authorKaseloo, Paulen
dc.contributor.authorAko, Ankrah A.en
dc.date.accessioned2020-07-23T16:47:47Zen
dc.date.available2020-07-23T16:47:47Zen
dc.date.issued2019-09-01en
dc.date.updated2020-07-23T16:47:38Zen
dc.description.abstractThere is a scarcity of information on antibiotic resistance in goats. To understand shedding of resistant Escherichia coli in pastured goats, we collected fecal samples from a mixed age cohort over a one-year period. No antibiotic had been used on the study animals one year prior to and during the study period. Resistant isolates were detected in all age groups and prevalence in goat kids was significantly higher than adults; 43–48% vs 8–25% respectively. The proportion of resistant isolates was higher when animals were congregated near handling facility than on pasture. Most isolates were resistant to tetracycline (51%) and streptomycin (30%), but also to antibiotics that had never been used on the farm; ampicillin (19%). TetB, bla-TEM, (aadA and strpA/strpB) genes were detected in 70%, 43%, (44% and 24%) of tetracycline, ampicillin, and streptomycin resistant isolates respectively. Resistant isolates also harbored virulent genes and some belonged to D and B2 phylogenetic groups. Thus, pastured goats, despite minimal exposure to antibiotics, are reservoirs of resistant E. coli that may contaminate the environment and food chain and spread resistant genes to pathogenic bacteria and some that are potential animal and human pathogens. Environmental sources may play a role in acquisition of resistant bacteria in pastured goats.en
dc.description.versionPublished versionen
dc.format.extent18 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 136 (Article number)en
dc.identifier.doihttps://doi.org/10.3390/antibiotics8030136en
dc.identifier.eissn2079-6382en
dc.identifier.issn2079-6382en
dc.identifier.issue3en
dc.identifier.otherantibiotics8030136 (PII)en
dc.identifier.pmid31480769en
dc.identifier.urihttp://hdl.handle.net/10919/99401en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectInfectious Diseasesen
dc.subjectPharmacology & Pharmacyen
dc.subjectantibiotic resistanceen
dc.subjectgoatsen
dc.subjectgoat kidsen
dc.subjectresistant genesen
dc.subjectvirulence genesen
dc.subjectphylogenetic groupingen
dc.subjectFECAL ESCHERICHIA-COLIen
dc.subjectANTIMICROBIAL RESISTANCEen
dc.subjectFOOD ANIMALSen
dc.subjectMULTIPLEX PCRen
dc.subjectCLASS-1 INTEGRONSen
dc.subjectVIRULENCE GENESen
dc.subjectBETA-LACTAMASESen
dc.subjectUNITED-STATESen
dc.subjectSTRAINSen
dc.subjectHUMANSen
dc.titleLongitudinal Shedding Patterns and Characterization of Antibiotic Resistant E. coli in Pastured Goats Using a Cohort Studyen
dc.title.serialAntibioticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2019-08-28en
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Virginia Cooperative Extensionen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/VSU Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/VSU Faculty/VSU Research Facultyen
pubs.organisational-group/Virginia Techen

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