A Cross Sectional Sampling Reveals Novel Coronaviruses in Bat Populations of Georgia

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dc.contributor.authorUrushadze, Lelaen
dc.contributor.authorBabuadze, Georgeen
dc.contributor.authorShi, Mangen
dc.contributor.authorEscobar, Luis E.en
dc.contributor.authorMauldin, Matthew R.en
dc.contributor.authorNatradeze, Ioseben
dc.contributor.authorMachablishvili, Annen
dc.contributor.authorKutateladze, Tamaren
dc.contributor.authorImnadze, Paataen
dc.contributor.authorNakazawa, Yoshinorien
dc.contributor.authorVelasco-Villa, Andresen
dc.coverage.countryGeorgiaen
dc.date.accessioned2022-01-11T13:48:40Zen
dc.date.available2022-01-11T13:48:40Zen
dc.date.issued2021-12-31en
dc.date.updated2022-01-10T14:38:17Zen
dc.description.abstractMammal-associated coronaviruses have a long evolutionary history across global bat populations, which makes them prone to be the most likely ancestral origins of coronavirus-associated epidemics and pandemics globally. Limited coronavirus research has occurred at the junction of Europe and Asia, thereby investigations in Georgia are critical to complete the coronavirus diversity map in the region. We conducted a cross-sectional coronavirus survey in bat populations at eight locations of Georgia, from July to October of 2014. We tested 188 anal swab samples, remains of previous pathogen discovery studies, for the presence of coronaviruses using end-point pan-coronavirus RT-PCR assays. Samples positive for a 440 bp amplicon were Sanger sequenced to infer coronavirus subgenus or species through phylogenetic reconstructions. Overall, we found a 24.5% positive rate, with 10.1% for <i>Alphacoronavirus</i> and 14.4% for <i>Betacoronavirus</i>. Albeit <i>R. euryale</i>, <i>R. ferrumequinum</i>, <i>M. blythii</i> and <i>M. emarginatus</i> were found infected with both CoV genera, we could not rule out CoV co-infection due to limitation of the sequencing method used and sample availability. Based on phylogenetic inferences and genetic distances at nucleotide and amino acid levels, we found one putative new subgenus and three new species of <i>Alphacoronavirus</i>, and two new species of <i>Betacoronavirus</i>.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationUrushadze, L.; Babuadze, G.; Shi, M.; Escobar, L.E.; Mauldin, M.R.; Natradeze, I.; Machablishvili, A.; Kutateladze, T.; Imnadze, P.; Nakazawa, Y.; Velasco-Villa, A. A Cross Sectional Sampling Reveals Novel Coronaviruses in Bat Populations of Georgia. Viruses 2021, 14, 72.en
dc.identifier.doihttps://doi.org/10.3390/v14010072en
dc.identifier.urihttp://hdl.handle.net/10919/107531en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcoronavirusen
dc.subjectbatsen
dc.subjectgeorgiaen
dc.subjecteastern europeen
dc.subjectphylogenyen
dc.subjectalphacoronavirusen
dc.subjectbetacoronavirusen
dc.titleA Cross Sectional Sampling Reveals Novel Coronaviruses in Bat Populations of Georgiaen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Journal Articles, Multidisciplinary Digital Publishing Institute (MDPI)
Destination Area: Global Systems Science (GSS)
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