Reducing Frost during Cryoimaging Using a Hygroscopic Ice Frame

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dc.contributor.authorLowery, Adam W.en
dc.contributor.authorAmbi, Ashwinen
dc.contributor.authorMiller, Lisa M.en
dc.contributor.authorBoreyko, Jonathan B.en
dc.date.accessioned2023-04-18T15:06:13Zen
dc.date.available2023-04-18T15:06:13Zen
dc.date.issued2022-12en
dc.description.abstractCryomicroscopy is commonly hampered by frost accumulation, reducing the visual clarity of the specimen. Pulling a vacuum or purging with nitrogen gas can greatly reduce the sample chamber's humidity, but at cryogenic temperatures, even minute concentrations of water vapor can still result in frost deposition. Here, a hygroscopic ice frame was created around the specimen to suppress frost growth during cryomicroscopy. Specifically, fluorescently tagged rat brain vessels were frozen on a silicon nitride window with an ice frame, and the luminescence of the fluorescent tag was improved by a factor of 6 compared to a similar specimen in only a nitrogen purge environment. These findings suggest that the simple implementation of a hygroscopic ice frame surrounding the specimen can substantially improve the visual clarity for cryomicroscopy, beyond that of a vacuum or nitrogen purge system.en
dc.description.notesThis work was supported by the Center of BioMolecular Structure (CBMS). CBMS is primarily supported by the National Institutes of Health, the National Institute of General Medical Sciences (NIGMS), through a Center Core P30 Grant (P30GM133893), and by the DOE Office of Biological and Environmental Research (DOE-BER) (KP1607011). The BioImaging Core within the CMBS was supported entirely by the DOE-BER.en
dc.description.sponsorshipCenter of BioMolecular Structure (CBMS); National Institutes of Health, the National Institute of General Medical Sciences (NIGMS), through a Center Core P30 Grant [P30GM133893]; DOE Office of Biological and Environmental Research (DOE-BER) [KP1607011]; DOE-BERen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1021/acsomega.2c03083en
dc.identifier.issue51en
dc.identifier.pmid36506191en
dc.identifier.urihttp://hdl.handle.net/10919/114542en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherAmerican Chemical Societyen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectX-ray-fluorescenceen
dc.subjectcryoelectron microscopyen
dc.subjectelectron-microscopyen
dc.subjectvapor-pressuresen
dc.subjectcryomicroscopyen
dc.subjectlighten
dc.subjectautofluorescenceen
dc.subjectwateren
dc.titleReducing Frost during Cryoimaging Using a Hygroscopic Ice Frameen
dc.title.serialACS Omegaen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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