Quantitative phase spectroscopy

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dc.contributor.authorRinehart, M.en
dc.contributor.authorZhu, Y.en
dc.contributor.authorWax, A.en
dc.contributor.departmentElectrical and Computer Engineeringen
dc.contributor.departmentCenter for Photonics Technologyen
dc.date.accessioned2017-03-05T17:55:37Zen
dc.date.available2017-03-05T17:55:37Zen
dc.date.issued2012-05-01en
dc.description.abstractQuantitative phase spectroscopy is presented as a novel method of measuring the wavelength-dependent refractive index of microscopic volumes. Light from a broadband source is filtered to an ~5 nm bandwidth and rapidly tuned across the visible spectrum in 1 nm increments by an acousto-optic tunable filter (AOTF). Quantitative phase images of semitransparent samples are recovered at each wavelength using off-axis interferometry and are processed to recover relative and absolute dispersion measurements. We demonstrate the utility of this approach by (i) spectrally averaging phase images to reduce coherent noise, (ii) measuring absorptive and dispersive features in microspheres, and (iii) quantifying bulk hemoglobin concentrations by absolute refractive index measurements. Considerations of using low coherence illumination and the extension of spectral techniques in quantitative phase measurements are discussed.en
dc.description.versionPublished versionen
dc.format.extent958 - 965 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1364/BOE.3.000958en
dc.identifier.eissn2156-7085en
dc.identifier.issue5en
dc.identifier.urihttp://hdl.handle.net/10919/75250en
dc.identifier.volume3en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/22567588en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject(090.6186) Spectral holographyen
dc.subject(110.4234) Multispectral and hyperspectral imagingen
dc.subject(120.5050) Phase measurementen
dc.subject(170.0170) Medical optics and biotechnologyen
dc.subject(180.3170) Interference microscopyen
dc.subject(300.0300) Spectroscopyen
dc.titleQuantitative phase spectroscopyen
dc.title.serialBiomedical Optics Expressen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/Electrical and Computer Engineeringen

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