Regulation of Sucrose non-Fermenting Related Kinase 1 genes in Arabidopsis thaliana

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dc.contributor.authorWilliams, Sarah P.en
dc.contributor.authorRangarajan, Padmaen
dc.contributor.authorDonahue, Janet L.en
dc.contributor.authorHess, Jenna S.en
dc.contributor.authorGillaspy, Glenda E.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2017-01-04T22:09:54Zen
dc.date.available2017-01-04T22:09:54Zen
dc.date.issued2014-07-10en
dc.description.abstractThe Sucrose non-Fermenting Related Kinase 1( SnRK1) proteins have been linked to regulation of energy and stress signaling in eukaryotes. In plants, there is a small SnRK1 gene family. While the SnRK1.1 gene has been well studied, the role other SnRK1 isoforms play in energy or stress signaling is less well understood. We used promoter:GUS analysis and found SnRK1.1is broadly expressed, while SnRK1.2 is spatially restricted. SnRK1.2 is expressed most abundantly in hydathodes, at the base of leaf primordia, and in vascular tissues within both shoots and roots. We examined the impact that sugars have on SnRK1 gene expression and found that trehalose induces SnRK1.2 expression. Given that the SnRK1.1 and SnRK1.2 proteins are very similar at the amino acid level, we sought to address whether SnRK1.2 is capable of re-programming growth and development as has been seen previously with SnRK1.1 over expression. While gain-of-function transgenic plants over expressing two different isoforms of SnRK1.1 flower late as seen previously in other SnRK1.1 over expressors, SnRK1.2 over expressors flower early. In addition, SnRK1.2 over expressors have increased leaf size and rosette diameter during. Early development, which is the opposite of SnRK1.1 over expressors. We also investigated whether SnRK1.2 was localized to similar subcellular compartments as SnRK1.1, and found that both accumulate in the nucleus and cytoplasm in transient expression assays. In addition, we found SnRK1.1 accumulates in small puncta that appear after a mechanical wounding stress. Together, these data suggest key differences in regulation of the SnRK1.1 and SnRK1.2 genes in plants, and highlights differences over expression of each gene has on the development of Arabidopsis.en
dc.description.versionPublished versionen
dc.identifier.doihttps://doi.org/10.3389/fpls.2014.00324en
dc.identifier.issn1664-462Xen
dc.identifier.urihttp://hdl.handle.net/10919/73944en
dc.identifier.volume5en
dc.language.isoenen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000339506700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 3.0 Unporteden
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectSnRK1en
dc.subjecttrehaloseen
dc.subjectsugar-sensingen
dc.subjectenergyen
dc.subjectgene expressionen
dc.titleRegulation of Sucrose non-Fermenting Related Kinase 1 genes in Arabidopsis thalianaen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen

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