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New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple

dc.contributor.authorMcHenry, Diana J.en
dc.contributor.authorAćimović, Srđan G.en
dc.date.accessioned2024-05-24T13:38:32Zen
dc.date.available2024-05-24T13:38:32Zen
dc.date.issued2024-04-27en
dc.date.updated2024-05-24T13:04:23Zen
dc.description.abstractBitter rot of apple is an economically important worldwide disease caused by different <i>Colletotrichum</i> species, depending on many factors such as climate, geography, other hosts, and crop management practices. Culture, morphology, and single-locus sequencing-based methods for identifying the <i>Colletotrichum</i> species are severely limited in effectiveness, while the multilocus sequence typing methods available for delineating species are costly, time-intensive, and require high expertise. We developed species-specific hydrolysis probe real-time PCR assays for the following nine <i>Colletotrichum</i> species causing bitter rot in the Mid-Atlantic U.S.A.: <i>C. fructicola</i>, <i>C. chrysophilum</i>, <i>C. noveboracense</i>, <i>C. gloeosporioides</i> s.s., <i>C. henanense</i>, <i>C. siamense</i> and <i>C. theobromicola</i> from the <i>C. gloeosporioides</i> species complex, and <i>C. fioriniae</i> and <i>C. nymphaeae</i> from the <i>C. acutatum</i> species complex. After searching 14 gene regions, we designed primers and probes in 5 of them for the nine target species. Four primer&ndash;probe set pairs were able to be duplexed. Sensitivity tests showed as little as 0.5 pg DNA were detectable. These real-time PCR assays will provide rapid and reliable identification of these key <i>Colletotrichum</i> species and will be critically important for studies aiming to elucidate their biology, epidemiology, and management on apples as the number one produced and consumed tree fruit in the U.S.A.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMcHenry, D.J.; Aćimović, S.G. New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple. Microorganisms 2024, 12, 878.en
dc.identifier.doihttps://doi.org/10.3390/microorganisms12050878en
dc.identifier.urihttps://hdl.handle.net/10919/119102en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectColletotrichumen
dc.subjectapple bitter roten
dc.subjectreal-time PCRen
dc.subjectpathogen diagnosticsen
dc.subjectrapid detectionen
dc.titleNew Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Appleen
dc.title.serialMicroorganismsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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