New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple
| dc.contributor.author | McHenry, Diana J. | en |
| dc.contributor.author | Aćimović, Srđan G. | en |
| dc.date.accessioned | 2024-05-24T13:38:32Z | en |
| dc.date.available | 2024-05-24T13:38:32Z | en |
| dc.date.issued | 2024-04-27 | en |
| dc.date.updated | 2024-05-24T13:04:23Z | en |
| dc.description.abstract | Bitter rot of apple is an economically important worldwide disease caused by different <i>Colletotrichum</i> species, depending on many factors such as climate, geography, other hosts, and crop management practices. Culture, morphology, and single-locus sequencing-based methods for identifying the <i>Colletotrichum</i> species are severely limited in effectiveness, while the multilocus sequence typing methods available for delineating species are costly, time-intensive, and require high expertise. We developed species-specific hydrolysis probe real-time PCR assays for the following nine <i>Colletotrichum</i> species causing bitter rot in the Mid-Atlantic U.S.A.: <i>C. fructicola</i>, <i>C. chrysophilum</i>, <i>C. noveboracense</i>, <i>C. gloeosporioides</i> s.s., <i>C. henanense</i>, <i>C. siamense</i> and <i>C. theobromicola</i> from the <i>C. gloeosporioides</i> species complex, and <i>C. fioriniae</i> and <i>C. nymphaeae</i> from the <i>C. acutatum</i> species complex. After searching 14 gene regions, we designed primers and probes in 5 of them for the nine target species. Four primer–probe set pairs were able to be duplexed. Sensitivity tests showed as little as 0.5 pg DNA were detectable. These real-time PCR assays will provide rapid and reliable identification of these key <i>Colletotrichum</i> species and will be critically important for studies aiming to elucidate their biology, epidemiology, and management on apples as the number one produced and consumed tree fruit in the U.S.A. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | McHenry, D.J.; Aćimović, S.G. New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple. Microorganisms 2024, 12, 878. | en |
| dc.identifier.doi | https://doi.org/10.3390/microorganisms12050878 | en |
| dc.identifier.uri | https://hdl.handle.net/10919/119102 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | Colletotrichum | en |
| dc.subject | apple bitter rot | en |
| dc.subject | real-time PCR | en |
| dc.subject | pathogen diagnostics | en |
| dc.subject | rapid detection | en |
| dc.title | New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple | en |
| dc.title.serial | Microorganisms | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |