Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation

dc.contributor.authorHolub, Meaghan N.en
dc.contributor.authorWahhab, Amandaen
dc.contributor.authorRouse, Joseph R.en
dc.contributor.authorDanner, Rebeccaen
dc.contributor.authorHackner, Lauren G.en
dc.contributor.authorDuris, Christine B.en
dc.contributor.authorMcClune, Mecaila E.en
dc.contributor.authorDressler, Jules M.en
dc.contributor.authorStrle, Klemenen
dc.contributor.authorJutras, Brandon L.en
dc.contributor.authorEdelstein, Adam I.en
dc.contributor.authorLochhead, Robert B.en
dc.description.abstractObjectives: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), and if its presence is associated with inflammation and patient reported outcomes. Methods: Intraoperative synovial tissue and synovial fluid samples were obtained from 56 patients undergoing primary TKA, none of whom had history of infection. PG in synovial tissue was detected by immunohistochemistry (IHC) and immunofluorescence microscopy (IFM). Synovial tissue inflammation and fibrosis were assessed by histopathology and synovial fluid cytokine quantification. Primary human fibroblasts isolated from arthritis synovial tissue were stimulated with PG to determine inflammatory cytokine response. Results: A total of 33/56 (59%) of primary TKA synovial tissue samples were positive for PG by IHC, and PG staining colocalized with markers of synovial macrophages and fibroblasts by IFM. Synovial tissue inflammation and elevated IL-6 in synovial fluid positively correlated with PG positivity. Primary human fibroblasts stimulated with PG secreted high levels of IL-6, consistent with ex vivo findings. Interestingly, we observed a significant inverse correlation between PG and age at time of TKA, indicating younger age at time of TKA was associated with higher PG levels. Conclusion: Peptidoglycan is commonly found in synovial tissue from patients undergoing TKA. Our data indicate that PG may play an important role in inflammatory synovitis, particularly in patients who undergo TKA at a relatively younger age.en
dc.description.versionPublished versionen
dc.identifier.citationArthritis Research & Therapy. 2024 Mar 27;26(1):77en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderThe Author(s)en
dc.titlePeptidoglycan in osteoarthritis synovial tissue is associated with joint inflammationen
dc.title.serialArthritis Research & Therapyen
dc.typeArticle - Refereeden
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
2.98 MB
Adobe Portable Document Format
Published version
License bundle
Now showing 1 - 1 of 1
1.5 KB
Item-specific license agreed upon to submission