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Identification of MOS9 as an interaction partner for chalcone synthase in the nucleus

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dc.contributor.authorWatkinson, Jonathan I.en
dc.contributor.authorBowerman, Peter A.en
dc.contributor.authorCrosby, Kevin C.en
dc.contributor.authorHildreth, Sherry B.en
dc.contributor.authorHelm, Richard F.en
dc.contributor.authorWinkel, Brenda S. J.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2019-01-18T19:04:14Zen
dc.date.available2019-01-18T19:04:14Zen
dc.date.issued2018-09-19en
dc.description.abstractPlant flavonoid metabolism has served as a platform for understanding a range of fundamental biological phenomena, including providing some of the early insights into the subcellular organization of metabolism. Evidence assembled over the past three decades points to the organization of the component enzymes as a membrane-associated complex centered on the entry-point enzyme, chalcone synthase (CHS), with flux into branch pathways controlled by competitive protein interactions. Flavonoid enzymes have also been found in the nucleus in a variety of plant species, raising the possibility of alternative, or moonlighting functions for these proteins in this compartment. Here, we present evidence that CHS interacts with MOS9, a nuclear-localized protein that has been linked to epigenetic control of R genes that mediate effector-triggered immunity. Overexpression of MOS9 results in a reduction of CHS transcript levels and a metabolite profile that substantially intersects with the effects of a null mutation in CHS. These results suggest that the MOS9–CHS interaction may point to a previously-unknown mechanism for controlling the expression of the highly dynamic flavonoid pathway.en
dc.description.sponsorshipThis work was supported by grants from the NSF Molecular Biochemistry (MCB-0445878), IGERT (DGE-0523658), and Arabidopsis 2010 (IOS-0820674) programs. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.extent20 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationWatkinson et al. (2018), Identification of MOS9 as an interaction partner for chalcone synthase in the nucleus. PeerJ 6:e5598; DOI 10.7717/peerj.5598en
dc.identifier.doihttps://doi.org/10.7717/peerj.5598en
dc.identifier.urihttp://hdl.handle.net/10919/86765en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherPeerJen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiochemistryen
dc.subjectCell Biologyen
dc.subjectPlant Scienceen
dc.subjectMOS9en
dc.subjectBiotic and abiotic stressen
dc.subjectChalcone synthaseen
dc.subjectNuclear localizationen
dc.subjectProtein interaction networksen
dc.subjectFlavonoid metabolismen
dc.titleIdentification of MOS9 as an interaction partner for chalcone synthase in the nucleusen
dc.title.serialPeerJen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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