Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms
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TR Number
Date
2007-02
Journal Title
Journal ISSN
Volume Title
Publisher
Optical Society of America
Abstract
We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.
Description
Keywords
superresolution optical-system, bandwidth product adaptation, shifting, digital holography, fluorescence microscopy, classical limit, image-formation, contrast
Citation
Guy Indebetouw, Yoshitaka Tada, Joseph Rosen, and Gary Brooker, "Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms," Appl. Opt. 46, 993-1000 (2007); doi: 10.1364/ao.46.000993