Mapping and Characterization of Phytophthora sojae and Soybean Mosaic Virus Resistance in Soybean

dc.contributor.authorTucker, Dominic M.en
dc.contributor.committeechairSaghai-Maroof, Mohammad A.en
dc.contributor.committeememberTolin, Sue A.en
dc.contributor.committeememberJelesko, John G.en
dc.contributor.committeememberRainey, Katy Martinen
dc.contributor.committeememberTyler, Brett M.en
dc.contributor.departmentCrop and Soil Environmental Sciencesen
dc.description.abstractPhytophthora sojae, the causal organism of stem and root rot, and <i>Soybean mosaic virus</i> (SMV) cause two of the most highly destructive diseases of soybean (<i>Glycine max</i> L. Merr). <i>P. sojae</i> can be managed either through deployment of race-specific resistance or through quantitative resistance termed partial resistance. In the current study, partial resistance to <i>P. sojae</i> was mapped in an interspecific recombinant inbred line (RIL) population of <i>Glycine max</i> by <i>Glycine soja</i>. One major quantitative trait loci (QTL) on molecular linkage group (MLG)-J (chromosome 16) and two minor QTL on MLG-I (chromosome 20) and -G (chromosome 18) were mapped using conventional molecular markers. Additionally, partial resistance to <i>P. sojae</i> was mapped in the same RIL population using single feature polymorphism (SFP) markers that further fine mapped the <i>P. sojae</i> QTL and identified potential candidate genes contributing to resistance. In a separate study, race-specific resistance was characterized in PI96983 discovering a potentially new allele of <i>Rps4</i> on MLG-G. Finally, using the newly available whole-genome shotgun sequence of soybean, <i>Rsv4</i> conferring resistance to strains of SMV known in the US, was localized to an approximately 100 kb region of sequence on chromosome 2 (MLG-D1B). Newly designed PCR-based markers permit for efficient selection of <i>Rsv4</i> by breeding programs. Identified candidate genes for <i>Rsv4</i> are discussed. Genomic resources developed in all of these studies provide breeders the tools necessary for developing durable resistance to both SMV and <i>P. sojae</i>.en
dc.description.degreePh. D.en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.subjectSingle feature polymorphismen
dc.subjectPhytophthora megaspermaen
dc.subjectDurable resistanceen
dc.subjectLate susceptibleen
dc.subjectSimple sequence repeaten
dc.subjectMarker-assisted selectionen
dc.titleMapping and Characterization of Phytophthora sojae and Soybean Mosaic Virus Resistance in Soybeanen
dc.type.dcmitypeTexten and Soil Environmental Sciencesen Polytechnic Institute and State Universityen D.en