Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments

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dc.contributor.authorKreutzberger, Mark A. B.en
dc.contributor.authorSobe, Richard C.en
dc.contributor.authorSauder, Amber B.en
dc.contributor.authorChatterjee, Sharanyaen
dc.contributor.authorPena, Alejandroen
dc.contributor.authorWang, Fengbinen
dc.contributor.authorGiron, Jorge A.en
dc.contributor.authorKiessling, Volkeren
dc.contributor.authorCosta, Tiago RD D.en
dc.contributor.authorConticello, Vincent P.en
dc.contributor.authorFrankel, Gaden
dc.contributor.authorKendall, Melissa M.en
dc.contributor.authorScharf, Birgit E.en
dc.contributor.authorEgelman, Edward H.en
dc.date.accessioned2024-01-22T13:39:55Zen
dc.date.available2024-01-22T13:39:55Zen
dc.date.issued2022-03-17en
dc.description.abstractFlagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in many bacteria and their structures and functions are not conserved. Here, we show the atomic cryo-electron microscopy structures for flagellar filaments from enterohemorrhagic Escherichia coli O157:H7, enteropathogenic E. coli O127:H6, Achromobacter, and Sinorhizobium meliloti, where the outer domains dimerize or tetramerize to form either a sheath or a screw-like surface. These dimers are formed by 180° rotations of half of the outer domains. The outer domain sheath (ODS) plays a role in bacterial motility by stabilizing an intermediate waveform and prolonging the tumbling of E. coli cells. Bacteria with these ODS and screw-like flagellar filaments are commonly found in soil and human intestinal environments of relatively high viscosity suggesting a role for the dimerization in these environments.en
dc.description.versionPublished versionen
dc.format.extent14 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 1422 (Article number)en
dc.identifier.doihttps://doi.org/10.1038/s41467-022-29069-yen
dc.identifier.eissn2041-1723en
dc.identifier.issn2041-1723en
dc.identifier.issue1en
dc.identifier.orcidScharf, Birgit [0000-0001-6271-8972]en
dc.identifier.other10.1038/s41467-022-29069-y (PII)en
dc.identifier.pmid35301306en
dc.identifier.urihttps://hdl.handle.net/10919/117445en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherNature Portfolioen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000770426200038&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject.meshFlagellaen
dc.subject.meshHumansen
dc.subject.meshBacteriaen
dc.subject.meshEscherichia colien
dc.subject.meshFlagellinen
dc.subject.meshSoilen
dc.subject.meshCryoelectron Microscopyen
dc.subject.meshDimerizationen
dc.subject.meshViscosityen
dc.titleFlagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environmentsen
dc.title.serialNature Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2022-02-24en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen

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