Deoxynivalenol interferes with intestinal motility via injuring the contractility of enteric smooth muscle cells: A novel hazard to the gastrointestinal tract by environmental toxins

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dc.contributor.authorJi, Xuen
dc.contributor.authorQiao, Yuen
dc.contributor.authorZheng, Weijiangen
dc.contributor.authorJiang, Honglinen
dc.contributor.authorYao, Wenen
dc.date.accessioned2021-12-14T18:00:10Zen
dc.date.available2021-12-14T18:00:10Zen
dc.date.issued2021-11en
dc.description.abstractDeoxynivalenol (DON) is a prevalent Fusarium mycotoxin, occurs predominantly in the global environment, especially in cereals, animal feed and food commodities. The widespread contamination causes a serious risk to human and animal health. DON usually impairs weight gain, which is presumably from its capacity to reduce feed intake by interfering with intestinal motility. To clarify the role of smooth muscle cells (SMCs) contractility intestinal motility and growth inhibition caused by DON, twelve weaned piglets were firstly divided into two groups to feed control or Fusarium mycotoxin-contaminated (MC) diet. Results showed that the final body weight, average daily gain and average daily feed intake were significantly reduced in piglets fed the MC diet. Exposure to the MC diet also significantly decreased the thickness of smooth muscle layer and SMCs contractile markers expression (myosin heavy chain 11, smooth muscle actin gamma 2, transgelin, calponin 1) in jejunum and ileum of piglets. Furthermore, oral DON supplementation (3 mg/kg body weight) to mice in six consecutive days could significantly inhibit the upper intestinal transit, impede normal defecation and downregulate SMCs contractile markers expression in small intestine. Finally, we generated a porcine enteric smooth muscle cell line (PISMC), and found that DON could depress its contractility by decreasing PISMC proliferation, migration and contractile markers expression. In conclusion, these findings in vivo and in vitro suggest that DON, as a common environmental toxin, can not only reduce proliferative and motile phenotype, but also decrease contractile apparatus components (contractile markers expression) in SMCs, which in turn influences SMCs contractility and then interferes with intestinal motility and growth performance.en
dc.description.notesThis study was supported by a grant from the Jiangsu Modern Agricultural (Live Pig) Industry Technology System, China (JATS [2020] 412), and Science and Technology Innovation Team Project of Anhui Academy of Agricultural Sciences, China (2021YL027). The authors also would like to thank the China Scholarship Council, China that offered Xu Ji a scholarship for his studying at Virginia Tech as a visiting scholar.en
dc.description.sponsorshipJiangsu Modern Agricultural (Live Pig) Industry Technology System, China [JATS [2020] 412]; Science and Technology Innovation Team Project of Anhui Academy of Agricultural Sciences, China [2021YL027]; China Scholarship Council, ChinaChina Scholarship Councilen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.ecoenv.2021.112656en
dc.identifier.eissn1090-2414en
dc.identifier.issn0147-6513en
dc.identifier.other112656en
dc.identifier.pmid34411815en
dc.identifier.urihttp://hdl.handle.net/10919/106984en
dc.identifier.volume224en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectEnvironmental toxinsen
dc.subjectDeoxynivalenolen
dc.subjectIntestinal motilityen
dc.subjectEnteric smooth muscle cellsen
dc.subjectContractilityen
dc.titleDeoxynivalenol interferes with intestinal motility via injuring the contractility of enteric smooth muscle cells: A novel hazard to the gastrointestinal tract by environmental toxinsen
dc.title.serialEcotoxicology and Environmental Safetyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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