Insights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variants

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dc.contributor.authorWright, R. Clayen
dc.contributor.authorZahler, Mollye L.en
dc.contributor.authorGerben, Stacey R.en
dc.contributor.authorNemhauser, Jennifer L.en
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2019-01-21T17:06:22Zen
dc.date.available2019-01-21T17:06:22Zen
dc.date.issued2017-10en
dc.date.updated2019-01-21T17:06:14Zen
dc.description.abstractThe evolution of complex body plans in land plants has been paralleled by gene duplication and divergence within nuclear auxin-signaling networks. A deep mechanistic understanding of auxin signaling proteins therefore may allow rational engineering of novel plant architectures. Toward that end, we analyzed natural variation in the auxin receptor F-box family of wild accessions of the reference plant Arabidopsis thaliana and used this information to populate a structure/function map. We employed a synthetic assay to identify natural hypermorphic F-box variants and then assayed auxin-associated phenotypes in accessions expressing these variants. To more directly measure the impact of the strongest variant in our synthetic assay on auxin sensitivity, we generated transgenic plants expressing this allele. Together, our findings link evolved sequence variation to altered molecular performance and auxin sensitivity. This approach demonstrates the potential for combining synthetic biology approaches with quantitative phenotypes to harness the wealth of available sequence information and guide future engineering efforts of diverse signaling pathways.en
dc.description.versionPublished versionen
dc.format.extentPages 583-591en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1534/genetics.117.300092en
dc.identifier.eissn1943-2631en
dc.identifier.issn0016-6731en
dc.identifier.issue2en
dc.identifier.orcidWright, Robert [0000-0001-7125-3943]en
dc.identifier.otherPMC5629325en
dc.identifier.othergenetics.117.300092 (PII)en
dc.identifier.pmid28760746en
dc.identifier.urihttp://hdl.handle.net/10919/86808en
dc.identifier.volume207en
dc.language.isoenen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/28760746en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectauxin-induced degradationen
dc.subjectnatural variationen
dc.subjectsynthetic biologyen
dc.subject0604 Geneticsen
dc.subjectDevelopmental Biologyen
dc.subject.meshArabidopsisen
dc.subject.meshIndoleacetic Acidsen
dc.subject.meshF-Box Proteinsen
dc.subject.meshReceptors, Cell Surfaceen
dc.subject.meshPlant Proteinsen
dc.subject.meshArabidopsis Proteinsen
dc.subject.meshEvolution, Molecularen
dc.subject.meshSignal Transductionen
dc.subject.meshAllelesen
dc.subject.meshGenetic Variationen
dc.titleInsights into the Evolution and Function of Auxin Signaling F-Box Proteins in Arabidopsis thaliana Through Synthetic Analysis of Natural Variantsen
dc.title.alternativeSynthetic analysis of natural variants yields insights into the evolution and function of auxin signaling F-box proteins in Arabidopsis thalianaen
dc.title.serialGeneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, Non-U.S. Gov'ten
dc.type.otherResearch Support, U.S. Gov't, Non-P.H.S.en
dc.type.otherResearch Support, N.I.H., Extramuralen
dcterms.dateAccepted2017-07-24en
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biological Systems Engineeringen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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