The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes

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dc.contributor.authorHaac, Mary Etnaen
dc.contributor.authorAnderson, Michelle A. E.en
dc.contributor.authorEggleston, Heatheren
dc.contributor.authorMyles, Kevin M.en
dc.contributor.authorAdelman, Zach N.en
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-04-12T14:55:18Zen
dc.date.available2019-04-12T14:55:18Zen
dc.date.issued2015-04-20en
dc.description.abstractAedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided by the dsRBPs Loquacious (Loqs-PB, Loqs-PD) and R2D2, respectively. However, this functional specialization has not been investigated in other dipterans. We were unable to detect Loqs-PD in Ae. aegypti; analysis of other dipteran genomes demonstrated that this isoform is not conserved outside of Drosophila. Overexpression experiments and small RNA sequencing following depletion of each dsRBP revealed that R2D2 and Loqs-PA cooperate non-redundantly in siRNA production, and that these proteins exhibit an inhibitory effect on miRNA levels. Conversely, Loqs-PB alone interacted with mosquito dicer-1 and was essential for full miRNA production. Mosquito Loqs interacted with both argonaute 1 and 2 in a manner independent of its interactions with dicer. We conclude that the functional specialization of Loqs-PD in Drosophila is a recently derived trait, and that in other dipterans, including the medically important mosquitoes, Loqs-PA participates in both the miRNA and endo-siRNA based pathways.en
dc.description.notesNational Institutes of Health [AI085091 to Z.A., GM072767 to E.S.]. Funding for open access charge: NIH [AI085091] and the Fralin Life Science Institute at Virginia Tech.en
dc.description.sponsorshipNational Institutes of Health [AI085091, GM072767]; Fralin Life Science Institute at Virginia Techen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/nar/gkv152en
dc.identifier.eissn1362-4962en
dc.identifier.issn0305-1048en
dc.identifier.issue7en
dc.identifier.pmid25765650en
dc.identifier.urihttp://hdl.handle.net/10919/88948en
dc.identifier.volume43en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectdisease vector mosquitosen
dc.subjectaedes-aegyptien
dc.subjectinfectious-diseasesen
dc.subjectgene-expressionen
dc.subjectyellow-feveren
dc.subjectdrosophilaen
dc.subjectsequencesen
dc.subjectsirnaen
dc.subjecttranscriptionen
dc.subjectmaturationen
dc.titleThe hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoesen
dc.title.serialNucleic Acids Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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