Identification of Candidate Iron Transporters From the ZIP/ZnT Gene Families in the Mosquito Aedes aegypti

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dc.contributor.authorTsujimoto, Hitoshien
dc.contributor.authorAnderson, Michelle A. E.en
dc.contributor.authorMyles, Kevin M.en
dc.contributor.authorAdelman, Zach N.en
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-10-29T17:26:59Zen
dc.date.available2019-10-29T17:26:59Zen
dc.date.issued2018-04-12en
dc.description.abstractMosquito-transmitted viral pathogens, such as dengue and Zika, afflict tens of thousands of people every year. These viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being Aedes aegypti. While vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. Although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquitoes lack homologs of the common iron transporter NRAMP. Using a novel cell-based screen, we identified two ZIP and one ZnT genes as candidate iron transporters in the mosquito A. aegypti, the vector of dengue, Zika, and chikungunya. We determined the organ-specific expression pattern of these genes at critical time points in early reproduction. The result indicates modulation of these genes upon blood feeding, especially a ZIP13 homolog that is highly up-regulated after blood feeding, suggesting its importance in iron mobilization during blood digestion and reproduction. Gene silencing resulted in differential iron accumulation in the midgut and ovaries. This study sets a foundation for further investigation of iron transport and control strategies of this viral vector.en
dc.description.notesThis study was supported by the National Institute of Allergies and Infectious Diseases of the National Institutes of Health under award number AI115138.en
dc.description.sponsorshipNational Institute of Allergies and Infectious Diseases of the National Institutes of Health [AI115138]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphys.2018.00380en
dc.identifier.issn1664-042Xen
dc.identifier.other380en
dc.identifier.pmid29706902en
dc.identifier.urihttp://hdl.handle.net/10919/95200en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAedesen
dc.subjectiron transporteren
dc.subjectZIPen
dc.subjectZnTen
dc.subjectferritinen
dc.titleIdentification of Candidate Iron Transporters From the ZIP/ZnT Gene Families in the Mosquito Aedes aegyptien
dc.title.serialFrontiers in Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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