Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii
| dc.contributor | Virginia Tech | en |
| dc.contributor.author | Cheng, Kun | en |
| dc.contributor.author | Zhang, Fei | en |
| dc.contributor.author | Sun, Fangfang | en |
| dc.contributor.author | Chen, Hongge | en |
| dc.contributor.author | Zhang, Y. H. Percival | en |
| dc.contributor.department | Biological Systems Engineering | en |
| dc.date.accessioned | 2017-03-13T17:13:41Z | en |
| dc.date.available | 2017-03-13T17:13:41Z | en |
| dc.date.issued | 2015-08-20 | en |
| dc.description.abstract | Biobattery, a kind of enzymatic fuel cells, can convert organic compounds (e.g., glucose, starch) to electricity in a closed system without moving parts. Inspired by natural starch metabolism catalyzed by starch phosphorylase, isoamylase is essential to debranch alpha-1,6-glycosidic bonds of starch, yielding linear amylodextrin – the best fuel for sugar-powered biobattery. However, there is no thermostable isoamylase stable enough for simultaneous starch gelatinization and enzymatic hydrolysis, different from the case of thermostable alpha-amylase. A putative isoamylase gene was mined from megagenomic database. The open reading frame ST0928 from a hyperthermophilic archaeron Sulfolobus tokodaii was cloned and expressed in E. coli. The recombinant protein was easily purified by heat precipitation at 80 °C for 30 min. This enzyme was characterized and required Mg²⁺ as an activator. This enzyme was the most stable isoamylase reported with a half lifetime of 200 min at 90 °C in the presence of 0.5 mM MgCl₂, suitable for simultaneous starch gelatinization and isoamylase hydrolysis. The cuvett-based air-breathing biobattery powered by isoamylase-treated starch exhibited nearly doubled power outputs than that powered by the same concentration starch solution, suggesting more glucose 1-phosphate generated. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1038/srep13184 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/76637 | en |
| dc.identifier.volume | 5 | en |
| dc.language.iso | en | en |
| dc.publisher | Nature | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.title | Doubling Power Output of Starch Biobattery Treated by the Most Thermostable Isoamylase from an Archaeon Sulfolobus tokodaii | en |
| dc.title.serial | Scientific Reports | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |
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