Control of sex myoblast migration in C. elegans
| dc.contributor.author | Zhang, Sihui | en |
| dc.contributor.committeechair | Kuhn, Jeffrey R. | en |
| dc.contributor.committeemember | Huckle, William R. | en |
| dc.contributor.committeemember | Cimini, Daniela | en |
| dc.contributor.committeemember | Banerjee, Diya | en |
| dc.contributor.department | Biological Sciences | en |
| dc.date.accessioned | 2015-01-24T07:00:18Z | en |
| dc.date.available | 2015-01-24T07:00:18Z | en |
| dc.date.issued | 2013-08-01 | en |
| dc.description.abstract | Cell migration is critical in generating complex animal forms during development; misregulation of migration contributes to pathological conditions such as cancer metastasis. Thanks to its easily traceable cell lineages in a transparent body and a compact genome accessible to a wealth of genetic manipulations, the use of the nematode C. elegans as a model system has greatly advanced our understanding of mechanisms governing cell migration conserved through higher organisms. Among several migration processes in C. elegans, sex myoblast (SM) migration is an attractive system that has a simple and well-defined migratory route along the ventral side from the posterior to the precise center of the gonad. A multitude of guidance mechanisms control SM migration, many of which are likely to be conserved in other migratory processes. Similar to vertebrate systems, C. elegans uses Rho family small GTPases to regulate the engine of cell motility, the actin cytoskeleton, in response to guidance cues. The differential utilizations of Rho GTPases in distinct processes in vivo remain a central question in the study of Rho GTPases. I investigated how Rho GTPases regulate different aspects of SM migration, and found that Cdc-42/CDC42 functions in the anteroposterior migration, whereas MIG-2/RhoG and CED-10/Rac1 control ventral restriction independently of FGF and SLIT/Robo signaling. The relative difficulty in perturbing SM migration using constitutively active Rho GTPases compared to other migration processes illustrates the robustness of the mechanisms that control SM migration. On a technical aspect, I established a nematode larval cell culture system that allows access to postembryonic cells. Compared to the flourishing genetic researches in C. elegans, there are few studies of molecules that also extend to the subcellular level in postembryonic development, mainly due to the lack of a larval cell culture system. I developed a novel method combining SDS-DTT presensitization of larval cuticles and subsequent pronase E digestion. My method efficiently isolates both low- and high-abundance cell types from all larval stages. This technical advance will not only facilitate studies such as regulation of actin dynamics with high-resolution microscopy, but is beginning to be used by researchers to tackle cell-type specific questions through profiling methods as gene expression analysis. | en |
| dc.description.degree | Ph. D. | en |
| dc.format.medium | ETD | en |
| dc.identifier.other | vt_gsexam:1353 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/51213 | en |
| dc.publisher | Virginia Tech | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject | cell migration | en |
| dc.subject | actin | en |
| dc.subject | Rho GTPase | en |
| dc.subject | C. elegans | en |
| dc.subject | sex myoblast | en |
| dc.title | Control of sex myoblast migration in C. elegans | en |
| dc.type | Dissertation | en |
| thesis.degree.discipline | Biological Sciences | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | doctoral | en |
| thesis.degree.name | Ph. D. | en |