Characterization of chitinase activity and gene expression in muskmelon seeds

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dc.contributor.authorWitmer, Xiaohongen
dc.contributor.authorNonogaki, Hiroyukien
dc.contributor.authorBeers, Eric P.en
dc.contributor.authorBradford, Kent J.en
dc.contributor.authorWelbaum, Gregory E.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessed2014-07-15en
dc.date.accessioned2014-07-21T15:49:45Zen
dc.date.available2014-07-21T15:49:45Zen
dc.date.issued2003-05en
dc.description.abstractChitinase is often produced in higher plants as a general defence response after wounding or pathogenic attack. Since germinating seeds are exposed to soil pathogens, the activity and expression of chitinase in muskmelon (Cucumis melo L.) seeds was investigated. One acidic and three basic chitinase isoforms were detected, beginning 40 d after anthesis in developing and fully mature seeds. Both acidic and basic chitinase isoforms were found in endosperm tissue during imbibition and after radicle emergence. Basic chitinase isoforms, but not acidic isoforms, were detected in the embryonic axes of imbibed seeds and in seeds before germination, indicating that chitinases are developmentally regulated in specific seed tissues. Two complete cDNAs, Cmchi1 and Cmchi2, were cloned from germinated muskmelon seeds and are predicted to encode chitinases that show 95% identity to a class III chitinase from cucumber (Cucumis sativus L.) and 61% identity to a class II chitinase from soybean (Glycine max L.), respectively. Southern blotting indicated that Cmchi2 was present only once in the muskmelon genome, while Cmchi1 may be present in one or two copies. Cmchi1 and Cmchi2 mRNAs were only detected in radicles of germinating seeds and in roots of mature plants, so additional genes other than Cmchi1 and Cmchi2 must be responsible for the chitinase activity in developing seeds. Salicylic acid and benzothiadiazole stimulated the expression of Cmchi1, but not Cmchi2, after radicle emergence. A putative role for chitinase in muskmelon seeds is defence against fungal pathogens.en
dc.description.sponsorshipSDA-BARD grant US-2422-94en
dc.description.sponsorshipUSDA Regional Research Project W-168en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationX. Witmer, H. Nonogaki, E.P. Beers, K.J. Bradford and G.E. Welbaum, "Characterization of chitinase activity and gene expression in muskmelon seeds," Seed Science Research, 13, pp 167-178, 2003. doi:10.1079/SSR2003134en
dc.identifier.doihttps://doi.org/10.1079/ssr2003134en
dc.identifier.issn0960-2585en
dc.identifier.urihttp://hdl.handle.net/10919/49661en
dc.identifier.urlhttp://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=705352&fulltextType=RA&fileId=S0960258503000163en
dc.language.isoenen
dc.publisherCambridge University Pressen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcucumis meloen
dc.subjectchitinaseen
dc.subjectdefence responseen
dc.subjectseed germinationen
dc.subjectsystemicen
dc.subjectacquired resistanceen
dc.subjectsystemic acquired-resistanceen
dc.subjectsomatic embryo mutanten
dc.subjectclass-i chitinaseen
dc.subjectcucumis-melo len
dc.subjectarabinogalactan proteinsen
dc.subjectwater relationsen
dc.subjectgerminationen
dc.subjectinductionen
dc.subjectendospermen
dc.subjectbeta-1,3-glucanaseen
dc.subjectplant sciencesen
dc.titleCharacterization of chitinase activity and gene expression in muskmelon seedsen
dc.title.serialSeed Science Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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