Abscisic acid ameliorates glucose tolerance and obesity-induced inflammation

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dc.contributor.authorGuri, Amir Josephen
dc.contributor.committeechairBassaganya-Riera, Josepen
dc.contributor.committeememberBarbeau, William E.en
dc.contributor.committeememberThye, Forrest W.en
dc.contributor.committeememberLiu, Dongminen
dc.contributor.committeememberHuckle, William R.en
dc.contributor.committeememberKingston, David G. I.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2014-03-14T20:17:52Zen
dc.date.adate2007-11-28en
dc.date.available2014-03-14T20:17:52Zen
dc.date.issued2007-10-19en
dc.date.rdate2007-11-28en
dc.date.sdate2007-11-02en
dc.description.abstractObesity is a disease characterized by chronic inflammation and the progressive loss in systemic insulin sensitivity. One of the more effective medications in the treatment of insulin resistance have been the thiazolidinediones (TZDs), which act through the nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma ). Due to the many side-effects of TZDs, our laboratory sought out a natural phytochemical, abscisic acid (ABA), with chemical similarities to TZDs. Our first study demonstrated that ABA activates PPARgamma in vitro and significantly ameliorates white adipose tissue (WAT) inflammation and glucose tolerance in db/db mice. We next further examined the effect of ABA on the phenotype of adipose tissue macrophages (ATMs). In doing so, we discovered two separate ATM populations which differed in their expression of the macrophage surface glycoprotein and maturation marker F4/80 (F4/80hi vs F4/80lo). Dietary ABA-supplementation significantly reduced F4/80hiCCR2+ ATMs and had no effect on the F4/80lo population. Utilizing a tissue-specific knockout generated through Cre-lox recombination, we were able to determine that this effect was dependent on PPARgamma in immune cells. To further characterize the differences between the ATM subsets that were affected by ABA, we performed a multi-organ assessment (i.e., WAT, skeletal muscle and liver) of the effect of diet-induced obesity on the phenotype of infiltrating macrophages and T cells into metabolic organs. Based on our new data, we formulated a model by which F4/80hiCCR2hi ATMs infiltrate WAT and ultimately induce a CD11c+ pro-inflammatory phenotype in the resident F4/80loCCR2lo subset. Ultimately, our findings provide evidence that ABA has potential as an alternative preventive intervention, expound the role of PPARgamma in immune cells and, in general, expand our knowledge concerning the immunopathogenesis of obesity-induced insulin resistance.en
dc.description.degreePh. D.en
dc.identifier.otheretd-11022007-120119en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11022007-120119/en
dc.identifier.urihttp://hdl.handle.net/10919/29433en
dc.publisherVirginia Techen
dc.relation.haspartAG_Dissertation.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectabscisic aciden
dc.subjectObesityen
dc.subjectmacrophageen
dc.subjectinflammationen
dc.subjectwhite adipose tissueen
dc.titleAbscisic acid ameliorates glucose tolerance and obesity-induced inflammationen
dc.typeDissertationen
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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