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Age related differences in phylogenetic diversity, prevalence of Shiga toxins, Intimin, Hemolysin genes and select serogroups of Escherichia coli from pastured meat goats detected in a longitudinal cohort study

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dc.contributor.authorNdegwa, Euniceen
dc.contributor.authorAlahmde, Aberen
dc.contributor.authorKim, Chyeren
dc.contributor.authorKaseloo, Paulen
dc.contributor.authorO'Brien, Dahliaen
dc.contributor.departmentVirginia Agricultural Experiment Stationen
dc.date.accessioned2021-09-09T12:38:04Zen
dc.date.available2021-09-09T12:38:04Zen
dc.date.issued2020-07-30en
dc.date.updated2021-09-09T12:38:01Zen
dc.description.abstractBackground: Little is known on significance, diversity and characteristics of gut E. coli in goats despite their importance as food animals globally. We characterized the temporal dynamics in diversity of E. coli in fecal samples from a cohort of goat kids and adult meat goats on pasture over a one-year period. Isolates were characterized based on phylogenetic grouping, virulence genes; shiga toxins 1 and 2 (Stx1&Stx2) (STEC), intimin (eaeA), hemolysin (hly) and select important sero-groups (026, 045, 0103, 0126 and 0146) using molecular methods. Results: A total of 516 E. coli isolates were screened. Prevalence of virulence genes and STEC was 65 and 56% respectively. Prevalence of virulence genes and STEC was significantly higher in goat kids less than six months (76% /66%) than adults (48% /28%). Isolates with virulence profiles of two or more genes were also higher in young goat kids (50%) than adults (20%). Entero-pathogenic E. coli (EPEC-eaeA gene only) were mostly from pre-weaned goat kids while hly gene only isolates were significantly higher in adults. The stx1, stx2 and hly genes peaked around weaning (60, 63 and 52%) respectively. Goats kids were mostly hosts to group D (59%) while adults older than one year had B1 (75%) isolates. Group D isolates were most abundant at weaning (64%) and diarrhea samples (74%). Group B2 isolates overall (6%) were mostly detected around weaning (63%) while A isolates were 4% overall. Twenty-four isolates belonged to sero-groups 026, 0103 and 0146 with 70% of the isolates detected around weaning. Nineteen of these isolates were STEC with most harboring the stx1/stx2/hly/eae (25%) profile. Most belonged to O26 sero-group (75%) and phylogroup D (75%). Conclusion: To our knowledge this is the first study to highlight longitudinal age related differences in E. coli phylogenetic diversity, abundance of virulence genes and select important sero-groups in goats. Differences detected suggest a possible role of age and weaning stress in influencing E. coli diversity in the gut of goats. The findings are relevant to both animal and public health to advise on further studies on caprine E. coli isolates as animal and human pathogens.en
dc.description.versionPublished versionen
dc.format.extent15 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 266 (Article number)en
dc.identifier.doihttps://doi.org/10.1186/s12917-020-02479-0en
dc.identifier.eissn1746-6148en
dc.identifier.issn1746-6148en
dc.identifier.issue1en
dc.identifier.orcidOBrien, Dahlia [0000-0002-3810-1734]en
dc.identifier.other10.1186/s12917-020-02479-0 (PII)en
dc.identifier.pmid32731899en
dc.identifier.urihttp://hdl.handle.net/10919/104957en
dc.identifier.volume16en
dc.language.isoenen
dc.publisherBMCen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000557549200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectVeterinary Sciencesen
dc.subjectGoatsen
dc.subjectAgeen
dc.subjectCohorten
dc.subjectVirulence genesen
dc.subjectPhylogenetic groupingen
dc.subjectE. colien
dc.subjectSerotypeen
dc.subjectENTEROPATHOGENIC ESCHERICHIA-COLIen
dc.subjectVIRULENCE GENESen
dc.subjectMULTIPLEX PCRen
dc.subjectGENOTYPIC DIVERSITYen
dc.subjectDIARRHEIC CALVESen
dc.subjectNEONATAL KIDSen
dc.subjectDAIRY-CATTLEen
dc.subjectIDENTIFICATIONen
dc.subjectSEROTYPESen
dc.subjectSTRAINSen
dc.subject0601 Biochemistry and Cell Biologyen
dc.subject0605 Microbiologyen
dc.subject0707 Veterinary Sciencesen
dc.subjectVeterinary Sciencesen
dc.subject.meshFecesen
dc.subject.meshAnimalsen
dc.subject.meshGoatsen
dc.subject.meshEscherichia colien
dc.subject.meshEscherichia coli Infectionsen
dc.subject.meshDiarrheaen
dc.subject.meshAdhesins, Bacterialen
dc.subject.meshEscherichia coli Proteinsen
dc.subject.meshShiga Toxinsen
dc.subject.meshCohort Studiesen
dc.subject.meshLongitudinal Studiesen
dc.subject.meshAge Factorsen
dc.subject.meshPhylogenyen
dc.subject.meshVirulenceen
dc.subject.meshWeaningen
dc.subject.meshFemaleen
dc.subject.meshMaleen
dc.subject.meshHemolysin Proteinsen
dc.subject.meshSerogroupen
dc.titleAge related differences in phylogenetic diversity, prevalence of Shiga toxins, Intimin, Hemolysin genes and select serogroups of Escherichia coli from pastured meat goats detected in a longitudinal cohort studyen
dc.title.serialBMC Veterinary Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2020-07-15en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Virginia Cooperative Extensionen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/VSU Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/VSU Faculty/VSU Extension Facultyen

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