Comparison of high performance liquid chromatographic, gas liquid chromatographic, and Saccharomyces uvarum methods for the determination of B{u2086} compounds
| dc.contributor.author | Lim, Kim L. | en |
| dc.contributor.department | Human Nutrition and Foods | en |
| dc.date.accessioned | 2017-01-30T21:25:01Z | en |
| dc.date.available | 2017-01-30T21:25:01Z | en |
| dc.date.issued | 1981 | en |
| dc.description.abstract | A high performance liquid chromatographic (HPLC) system consisting of an acetonitrile/phosphate buffer, a Spherisorb ODS column, and UV detector was used to separate the B₆ compounds pyridoxol, pyridoxal, pyridoxamine, and 4'-deoxypyridoxine. A gas chromatography (GC) equipped with a ⁶³Ni electron capture (EC) detector was used to separate the N-methyl-bis-trif luoroacetamide derivatives of the B₆ compounds on a l.54m x 2mm i.d. glass column packed with 10% SP2100 on Supelcoport 90-100 mesh at 125°C and an inlet pressure of 40 psig. Clean and successful separations of all the B₆ forms were obtained by HPLC and GC-EC. Total B₆ values as determined by HPLC and GC-EC for all 3 foods were higher than the corresponding total B₆ values as determined by S. uvarum assay. Several of the B₆ vitamer values for foods obtained by GC-EC agree with corresponding values obtained by HPLC. The HPLC method seemed to be the most satisfactory of the 3 methods for the quantitation of B₆ vitamers in foods and has the following advantages: increased sensitivity, method simplicity, and good precision. | en |
| dc.description.degree | Ph. D. | en |
| dc.format.extent | xi, 128, [2] leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/74819 | en |
| dc.language.iso | en_US | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 7974262 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V856 1981.L545 | en |
| dc.subject.lcsh | Vitamin B6 | en |
| dc.title | Comparison of high performance liquid chromatographic, gas liquid chromatographic, and Saccharomyces uvarum methods for the determination of B{u2086} compounds | en |
| dc.type | Dissertation | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Human Nutrition and Foods | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | doctoral | en |
| thesis.degree.name | Ph. D. | en |
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