The application of planar optical waveguides to absorption spectrometry in flow injection analysis

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dc.contributor.authorChoquette, Steven Josephen
dc.contributor.committeechairDessy, Raymond E.en
dc.contributor.committeememberWard, Thomas C.en
dc.contributor.committeememberMason, John G.en
dc.contributor.committeememberDillard, John G.en
dc.contributor.committeememberLong, G.L.en
dc.contributor.departmentChemistryen
dc.date.accessioned2015-06-29T22:07:11Zen
dc.date.available2015-06-29T22:07:11Zen
dc.date.issued1988en
dc.description.abstractAttenuated total reflection techniques have been used extensively as analytical tools for the analysis of thin films and analytes imbedded in complex scattering matrices. However they have not been commonly utilized as detectors in common analytical techniques such as Flow Injection Analysis because of their relatively low sensitivity. The feasibility of using a thin film planar waveguide as an absorption sensor in the Flow Injection Analysis of Urea was investigated. Urea was hydrolyzed to ammonia and carbon dioxide with the enzyme Urease. The ammonia produced was quantitated colorimetrically using Berthelot’s reaction. The reaction product, indophenol blue, was detected using the combination planar waveguide 9.2 microliter flow cell sensor. The planar waveguides used had 2 to 3 orders of magnitude greater sensitivity than typical internal reflection elements. The analytical working range obtained for urea determinations was from 0 to 20 mM urea at a rate of 30 samples per hour. A description of the investigation and the various factors involved in designing and optimizing a planar waveguide for absorption spectrometry is included.en
dc.description.degreePh. D.en
dc.format.extentxiii, 239 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/53932en
dc.language.isoen_USen
dc.publisherVirginia Polytechnic Institute and State Universityen
dc.relation.isformatofOCLC# 17720651en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1988.C567en
dc.subject.lcshWave guidesen
dc.subject.lcshAbsorption spectraen
dc.titleThe application of planar optical waveguides to absorption spectrometry in flow injection analysisen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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