Development of defined media for motility and for growth of Spirillum volutans, with special reference to biological monitoring of pollutants and to obligate microaerophilism of bacteria

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dc.contributor.authorBowdre, Jean Handyen
dc.contributor.committeechairKrieg, Noel R.en
dc.contributor.committeememberPaterson, Robert A.en
dc.contributor.committeememberSmibert, Robert M.en
dc.contributor.committeememberWilkins, Tracy D.en
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2014-03-14T21:11:44Zen
dc.date.adate2010-06-02en
dc.date.available2014-03-14T21:11:44Zen
dc.date.issued1975-05-05en
dc.date.rdate2010-06-02en
dc.date.sdate2010-06-02en
dc.description.abstractTwo investigations were pursued in this study. One was a possible application of the motility of Spirillum volutans to detection of pollutants in industrial effluent for the purpose of in-plant biological monitoring. The other was a nutritional study of the organism with emphasis on its obligate microaerophilism. In the pollution monitoring project, flagellar uncoordination in S. volutans was studied as a bioindicator of toxicity. Uncoordination is produced by a variety of agents. Cells displaying normal motility show frequent reversal of direction, accompanied by reversal of orientation of their bipolar fascicles of flagella. In uncoordinated cells, the flagella at opposite poles oppose each other and the cell cannot swim, although the flagella remain active. A defined motility test medium was devised in an attempt to maximize the sensitivity of the uncoordination response to potential pollutants. Suspensions of S. volutans in this medium responded to a variety of agents, including metal ions (1-3 ppm), cetyl pyridinium chloride (1 ppm), hydrazine (10 ppm), i-naphthol (3 ppm), and others. Effective concentrations ranged up to several per cent for the alcohols tested. The response was immediate.en
dc.description.degreePh. D.en
dc.format.extentix, 147 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06022010-020410en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06022010-020410/en
dc.identifier.urihttp://hdl.handle.net/10919/37938en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1975.B693.pdfen
dc.relation.isformatofOCLC# 40132550en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectmotilityen
dc.subjectNutritionen
dc.subject.lccLD5655.V856 1975.B693en
dc.titleDevelopment of defined media for motility and for growth of Spirillum volutans, with special reference to biological monitoring of pollutants and to obligate microaerophilism of bacteriaen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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