Antioxidant activity of Mn-salophen complex and its effects on antioxidant enzymes in Escherichia coli

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dc.contributor.authorLiu, Zheng-Xianen
dc.contributor.committeechairGregory, Eugene M.en
dc.contributor.committeememberBevan, David R.en
dc.contributor.committeememberBunce, George Edwinen
dc.contributor.committeememberHess, John L.en
dc.contributor.committeememberMisra, Hara P.en
dc.contributor.departmentBiochemistry and Anaerobic Microbiologyen
dc.date.accessioned2014-03-14T21:21:51Zen
dc.date.adate2005-10-20en
dc.date.available2014-03-14T21:21:51Zen
dc.date.issued1994-11-05en
dc.date.rdate2005-10-20en
dc.date.sdate2005-10-20en
dc.description.abstractMn-salophen complex with superoxide-scavenging activity was prepared from manganese(III) acetate dihydrate and salophen in ethanol. Visible absorption spectrum of the red-brown solution exhibited a broad absorption band at 430 - 450 nm with two shoulders between 500 and 600 nm which were absent with either salophen or manganic acetate alone. Titration of salophen with manganese(III) was consistent with a 1:1 Mn to salophen stoichiometry of the complex based on changes in the absorbance at 500 nm or of superoxide scavenging activity. The SOD-like activity of the complex in the xanthine-xanthine oxidase/cytochrome <i>c</i> assay was 1450 units/mg salophen. The SOD activity of the complex was suppressed 50% in the presence of EDTA (1 mM), but was not altered in the presence of bovine serum albumin (1 mg/ml) or crude protein extract of <i>E. coli</i> QC779 <i>sodA sodB</i> (1 mg/ml). <i>E. coli</i> QC779 <i>sodA sodB</i> grew scantily after an 8 hour lag phase in aerobic M63 glucose minimal medium.en
dc.description.degreePh. D.en
dc.format.extentxiii, 154 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10202005-102838en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10202005-102838/en
dc.identifier.urihttp://hdl.handle.net/10919/40046en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1994.L597.pdfen
dc.relation.isformatofOCLC# 32793733en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1994.L597en
dc.subject.lcshAntioxidantsen
dc.subject.lcshEscherichia coli -- Physiologyen
dc.subject.lcshSuperoxide dismutaseen
dc.titleAntioxidant activity of Mn-salophen complex and its effects on antioxidant enzymes in Escherichia colien
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Anaerobic Microbiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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