Genetic Reporter System for Positioning of Proteins at the Bacterial Pole

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dc.contributor.authorFixen, Kathryn R.en
dc.contributor.authorJanakiraman, Anuradhaen
dc.contributor.authorGarrity, Seanen
dc.contributor.authorSlade, Daniel J.en
dc.contributor.authorGray, Andrew N.en
dc.contributor.authorKarahan, Nilayen
dc.contributor.authorHochschild, Annen
dc.contributor.authorGoldberg, Marcia B.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentCenter for Drug Discoveryen
dc.date.accessioned2016-11-09T02:58:41Zen
dc.date.available2016-11-09T02:58:41Zen
dc.date.issued2012en
dc.description.abstractSpatial organization within bacteria is fundamental to many cellular processes, although the basic mechanisms underlying localization of proteins to specific sites within bacteria are poorly understood. The study of protein positioning has been limited by a paucity of methods that allow rapid large-scale screening for mutants in which protein positioning is altered. We developed a genetic reporter system for protein localization to the pole within the bacterial cytoplasm that allows saturation screening for mutants in Escherichia coli in which protein localization is altered. Utilizing this system, we identify proteins required for proper positioning of the Shigella autotransporter IcsA. Autotransporters, widely distributed bacterial virulence proteins, are secreted at the bacterial pole. We show that the conserved cell division protein FtsQ is required for localization of IcsA and other autotransporters to the pole. We demonstrate further that this system can be applied to the study of proteins other than autotransporters that display polar positioning within bacterial cells.en
dc.description.versionPublished versionen
dc.identifier.doihttps://doi.org/10.1128/mBio.00251-11en
dc.identifier.issn2150-7511en
dc.identifier.issue2en
dc.identifier.urihttp://hdl.handle.net/10919/73402en
dc.identifier.volume3en
dc.language.isoenen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305297100022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.titleGenetic Reporter System for Positioning of Proteins at the Bacterial Poleen
dc.title.serialmBioen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
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