Characterization and regulation of the speA gene in Escherichia coli

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dc.contributor.authorMoore, Robert C.en
dc.contributor.committeechairBoyle, Stephen M.en
dc.contributor.committeememberDean, Dennis R.en
dc.contributor.committeememberJohnson, John L.en
dc.contributor.committeememberLacy, George H.en
dc.contributor.committeememberLarson, Timothy J.en
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T21:19:04Zen
dc.date.adate2005-09-20en
dc.date.available2014-03-14T21:19:04Zen
dc.date.issued1990en
dc.date.rdate2005-09-20en
dc.date.sdate2005-09-20en
dc.description.abstractIn Escherichia coli, the speA gene encodes biosynthetic arginine decarboxylase (ADC), the first enzyme in a putrescine biosynthetic pathway. ADC converts arginine to agmatine, which is hydrolyzed by agmatine ureohydrolase, encoded by the speB gene, to putrescine and urea. ADC is negatively regulated by mechanisms requiring either cAMP and cAMP receptor protein (CRP) or putrescine. A 3,236 base pair (bp) BalI-AccI restriction fragment derived from plasmid pKA5, which contains a 7.5 kilobase (kb) E. coli genomic fragment in pBR322, was subcloned into pGEM-3Z to produce plasmids pRM15 and pRM59. Both pRM15 and pRM59 overexpress ADC and the DNA sequence of the BalI-AccI fragment in each plasmid was determined. A 2,119 bp restriction fragment containing 730 bp 5’ to speA, the speA promoter, and 1,389 bp (463 amino acids) of the 5’-end of speA was used to construct transcriptional (pRM161 and pRM162) and translational (pRM65) speA-lacZ fusion plasmids. The presence of the predicted 160,000 and 157,000 dalton ADCen
dc.description.degreePh. D.en
dc.format.extentxi, 101 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-09202005-090951en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-09202005-090951/en
dc.identifier.urihttp://hdl.handle.net/10919/39408en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1990.M667.pdfen
dc.relation.isformatofOCLC# 23663445en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1990.M667en
dc.subject.lcshEscherichia colien
dc.subject.lcshPolyaminesen
dc.titleCharacterization and regulation of the speA gene in Escherichia colien
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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