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Dual effects of obesity on satellite cells and muscle regeneration

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dc.contributor.authorGeiger, Ashley E.en
dc.contributor.authorDaughtry, Morgan R.en
dc.contributor.authorYen, Con-Ningen
dc.contributor.authorKirkpatrick, Laila T.en
dc.contributor.authorShi, Haoen
dc.contributor.authorGerrard, David E.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2020-10-09T14:09:08Zen
dc.date.available2020-10-09T14:09:08Zen
dc.date.issued2020-08en
dc.description.abstractObesity is a complex metabolic disorder that often leads to a decrease in insulin sensitivity, chronic inflammation, and overall decline in human health and well-being. In mouse skeletal muscle, obesity has been shown to impair muscle regeneration after injury; however, the mechanism underlying these changes has yet to be determined. To test whether there is a negative impact of obesity on satellite cell (SC) decisions and behaviors, we fed C57BL/6 mice normal chow (NC, control) or a high-fat diet (HFD) for 10 weeks and performed SC proliferation and differentiation assays in vitro. SCs from HFD mice formed colonies with smaller size (p < .001) compared to those from NC mice, and this decreased proliferation was confirmed (p < .05) by BrdU incorporation. Moreover, in vitro assays showed that HFD SCs exhibited diminished (p < .001) fusion capacity compared to NC SCs. In single fiber explants, a higher ratio of SCs experienced apoptotic events (p < .001) in HFD mice compared to that of NC-fed mice. In vivo lineage tracing using H2B-GFP mice showed that SCs from HFD treatment also cycled faster (p < .001) than their NC counterparts. In spite of all these autonomous cellular effects, obesity as triggered by high-fat feeding did not significantly impair muscle regeneration in vivo, as reflected by the comparable cross-sectional area (p > .05) of the regenerating fibers in HFD and NC muscles, suggesting that other factors may mitigate the negative impact of obesity on SCs properties.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.14814/phy2.14511en
dc.identifier.issn2051-817Xen
dc.identifier.issue15en
dc.identifier.othere14511en
dc.identifier.pmid32776502en
dc.identifier.urihttp://hdl.handle.net/10919/100319en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmuscle regenerationen
dc.subjectObesityen
dc.subjectsatellite cellen
dc.titleDual effects of obesity on satellite cells and muscle regenerationen
dc.title.serialPhysiological Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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