Enzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous system

Simple item page
dc.contributor.authorYang, Bingen
dc.contributor.authorQin, Chaoen
dc.contributor.authorHu, Xiaojieen
dc.contributor.authorXia, Kangen
dc.contributor.authorLu, Chaoen
dc.contributor.authorGudda, Fredrick Owinoen
dc.contributor.authorMa, Zhaoen
dc.contributor.authorGao, Yanzhengen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-02-10T14:12:11Zen
dc.date.available2020-02-10T14:12:11Zen
dc.date.issued2019-11en
dc.description.abstractThe persistence of extracellular DNA (eDNA) is crucial for ensuring species diversity and ecological function in aquatic systems. However, scarce information exists about the impact of pesticides on eDNA, although they often co-exist in the aquatic environment. Using a variety of spectroscopic analyses, eDNA degradation and the associated alterations in DNA secondary structure was investigated by exposing DNase I to tested DNA in the presence of chlorpyrifos, a commonly used organophosphate pesticide. Molecular dynamics simulation was used to explore the weak interactions between the tested DNA and chlorpyrifos. The results indicated that chlorpyrifos significantly enhanced DNA degradation without affecting the enzyme activity of DNase I in an aqueous system. Spectroscopic experiments confirmed that chlorpyrifos and the analog chlorpyrifos-methyl could bind with DNA to cause the bases noncovalent stacking interaction. Molecular simulations further demonstrated that pesticide binding with DNA molecules caused widening of the DNA grooves and destruction of the hydrated layer, which enhanced DNA degradation. The findings presented herein provide novel insight into the genotoxicity and ecotoxicity of chlorpyrifos and chlorpyrifos-methyl, as well as their impacts on DNA persistence in aquatic environments.en
dc.description.notesThis work was supported by the National Natural Science Foundation of China (41877125, 41771523), and the Jiangsu Provincial Key Research and Development Program, China (BE2017718). We are grateful for computation resources from the High-Performance Computing System at National Engineering Laboratory of Soil Pollution Control and Remediation Technologies, CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences.en
dc.description.sponsorshipNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [41877125, 41771523]; Jiangsu Provincial Key Research and Development Program, China [BE2017718]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.envint.2019.105087en
dc.identifier.eissn1873-6750en
dc.identifier.issn0160-4120en
dc.identifier.otherUNSP 105087en
dc.identifier.pmid31430607en
dc.identifier.urihttp://hdl.handle.net/10919/96771en
dc.identifier.volume132en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectExtracellular DNAen
dc.subjectDeoxyribonuclease I (DNase I)en
dc.subjectOrganophosphorusen
dc.subjectPesticidesen
dc.subjectSpectroscopyen
dc.subjectMolecular dynamicsen
dc.titleEnzymatic degradation of extracellular DNA exposed to chlorpyrifos and chlorpyrifos-methyl in an aqueous systemen
dc.title.serialEnvironment Internationalen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S0160412019316150-main.pdf
Size:
3.36 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Scholarly Works, School of Plant and Environmental Sciences