An expanding role for purine uptake permease-like transporters in plant secondary metabolism

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dc.contributor.authorJelesko, John G.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-05-01T14:16:48Zen
dc.date.available2019-05-01T14:16:48Zen
dc.date.issued2012en
dc.description.abstractFor the past decade, our understanding of the plant purine uptake permease (PUP) transporter family was primarily oriented on purine nucleobase substrates and their tissue-specific expression patterns in Arabidopsis. However, a tobacco PUP-like homolog demonstrating nicotine uptake permease activity was recently shown to affect both nicotine metabolism and root cell growth. These new findings expand the physiological role for PUP-like transporters to include plant secondary metabolism. Molecular evolution analyses of PUP-like transporters indicate they are distinct group within an ancient super family of drug and metabolite transporters (DMTs). The PUP-like family originated during terrestrial plant evolution sometime between the bryophytes and the lycophytes. A phylogenetic analysis indicates that the PUP-like transporters were likely derived from a pre-existing nucleotide-sugar transporter family within the DMT super family. Within the lycophyte Selaginella, there are three paralogous groups of PUP-like transporters. One of the three PUP-like paralogous groups showed an extensive pattern of gene duplication and diversification within the angiosperm lineage, whereas the more ancestral PUP-like paralogous groups did not. Biochemical characterization of four closely related PUP-like paralogs together with model-based phylogenetic analyses indicate both subfunctionalization and neofunctionalization during the molecular evolution of angiosperm PUP-like transporters. These findings suggest that members of the PUP-like family of DMT transporters are likely involved in diverse primary and secondary plant metabolic pathways.en
dc.description.notesAlex Weisberg provided scripting to reformat tree taxa labels and Sarah Kashanian assigned TC codes to tree taxa labels. Dr. Angus Murphy provided helpful comments during the early drafting of the manuscript. I am also grateful for the helpful suggestions of the reviewers. This work was supported by a USDA award administered through the Virginia Agriculture and Experiment Station Biodesign and Bioprocessing Research Center to John G. Jelesko.en
dc.description.sponsorshipUSDAen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2012.00078en
dc.identifier.eissn1664-462Xen
dc.identifier.other78en
dc.identifier.pmid22639664en
dc.identifier.urihttp://hdl.handle.net/10919/89330en
dc.identifier.volume3en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectadenineen
dc.subjectalkaloiden
dc.subjectevolutionen
dc.subjectnicotineen
dc.subjecttransporten
dc.titleAn expanding role for purine uptake permease-like transporters in plant secondary metabolismen
dc.title.serialFrontiers In Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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