RNA interference suppression of AGAMOUS and SEEDSTICK alters floral organ identity and impairs floral organ determinacy, ovule differentiation, and seed-hair development in Populus

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dc.contributor.authorLu, Haiweien
dc.contributor.authorKlocko, Amy L.en
dc.contributor.authorBrunner, Amy M.en
dc.contributor.authorMa, Cathleenen
dc.contributor.authorMagnuson, Anna C.en
dc.contributor.authorHowe, Glenn T.en
dc.contributor.authorAn, Xinminen
dc.contributor.authorStrauss, Steven H.en
dc.contributor.departmentForest Resources and Environmental Conservationen
dc.date.accessioned2019-08-29T17:12:32Zen
dc.date.available2019-08-29T17:12:32Zen
dc.date.issued2019-04en
dc.description.abstractThe role of the floral homeotic gene AGAMOUS (AG) and its close homologues in development of anemophilous, unisexual catkins has not previously been studied. We transformed two RNA interference (RNAi) constructs, PTG and its matrix-attachment-region flanked version MPG, into the early-flowering female poplar clone 6K10 (Populus alba) to suppress the expression of its two duplicate AG orthologues. By early 2018, six out of 22 flowering PTG events and 11 out of 12 flowering MPG events showed modified floral phenotypes in a field trial in Oregon, USA. Flowers in catkins from modified events had carpel-inside-carpel' phenotypes. Complete disruption of seed production was observed in seven events, and sterile anther-like organs in 10 events. Events with strong co-suppression of both the two AG and two SEEDSTICK (STK) paralogues lacked both seeds and associated seed hairs. Alterations in all of the modified floral phenotypes were stable over 4yr of study. Trees from floral-modified events did not differ significantly (P<0.05) from nonmodified transgenic or nontransgenic controls in biomass growth or leaf morphology. AG and STK genes show strong conservation of gene function during poplar catkin development and are promising targets for genetic containment of exotic or genetically engineered trees.en
dc.description.notesThis project was supported by the USDA Biotechnology Risk Assessment Research Grants Program, competitive grant 2011-68005-30407 - National Institute of Food and Agriculture and Agricultural Research Service, National Science Foundation I/UCRC Center for Advanced Forestry (grant 0736283), USDA-BRAG (grant 2010-33522-21736), USDA-IFAS (grant OREZ-FS-671-R), and the TBGRC industrial cooperative at Oregon State University. We thank Dr Maurizio Sabatti (Tuscia University, Viterbo, Italy) for providing the female poplar clone 6K10, Ariel Muldoon (OSU consulting statistician) for advice on statistical analysis and comments on the related text in the Materials and Methods section, and several OSU undergraduate students who took part in field maintenance and vegetative data collection.en
dc.description.sponsorshipUSDA Biotechnology Risk Assessment Research Grants Program - National Institute of Food and Agriculture and Agricultural Research Service, National Science Foundation I/UCRC Center for Advanced Forestry [2011-68005-30407, 0736283]; USDA-BRAG [2010-33522-21736]; USDA-IFAS [OREZ-FS-671-R]; TBGRC industrial cooperative at Oregon State Universityen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/nph.15648en
dc.identifier.eissn1469-8137en
dc.identifier.issn0028-646Xen
dc.identifier.issue2en
dc.identifier.pmid30565259en
dc.identifier.urihttp://hdl.handle.net/10919/93303en
dc.identifier.volume222en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAGAMOUSen
dc.subjectcontainmenten
dc.subjectfloweringen
dc.subjectmatrix attachment regionen
dc.subjectPopulusen
dc.subjectRNAien
dc.subjectSEEDSTICKen
dc.subjectsterilityen
dc.titleRNA interference suppression of AGAMOUS and SEEDSTICK alters floral organ identity and impairs floral organ determinacy, ovule differentiation, and seed-hair development in Populusen
dc.title.serialNew Phytologisten
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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