A standard cytogenetic photomap for the mosquito Anopheles stephensi (Diptera : Culicidae): Application for physical mapping

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dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorXia, Aien
dc.contributor.authorMcAlister, Sarah I.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.departmentEntomologyen
dc.date.accessed2015-12-17en
dc.date.accessioned2016-02-28T22:29:49Zen
dc.date.available2016-02-28T22:29:49Zen
dc.date.issued2006-09en
dc.description.abstractTo facilitate physical genome mapping, we have developed a new cytogenetic photomap for Anopheles stephensi (Liston) (Diptera: Culicidae), an important malaria vector in Asia. The high-resolution images of the ovarian polytene chromosomes have been straightened and divided by numbered divisions and lettered subdivisions. The exact chromosomal locations of eight DNA probes have been determined by fluorescent in situ hybridization. Using the DNA sequences, we have established correspondence between chromosomal arms among An. stephensi, Anopheles gambiae (Patton), and Anopheles funestus (Giles). The results support previous cytogenetic observations of arm translocations taking place during diversification of the species. To make the cytogenetic map useful for population genetics studies, we have indicated the chromosomal positions for the breakpoints of 19 polymorpbic inversions.en
dc.description.sponsorshipVirginia Agricultural Experiment Station‏en
dc.format.extent6 p.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSharakhova, M. V., Xia, A., McAlister, S. I., & Sharakhov, I. V. (2006). A Standard Cytogenetic Photomap for the Mosquito Anopheles stephensi (Diptera: Culicidae): Application for Physical Mapping. Journal of Medical Entomology, 43(5), 861-866. doi:10.1093/jmedent/43.5.86en
dc.identifier.doihttps://doi.org/10.1093/jmedent/43.5.861en
dc.identifier.issn0022-2585en
dc.identifier.issue5en
dc.identifier.other861full.pdfen
dc.identifier.urihttp://hdl.handle.net/10919/64880en
dc.identifier.urlhttp://jme.oxfordjournals.org/content/43/5/861en
dc.identifier.volume43en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.holderOxford University Pressen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectAnopheles stephensien
dc.subjectCytogenetic photomapen
dc.subjectPolytene chromosomesen
dc.subjectFluorescent in situ hybridizationen
dc.titleA standard cytogenetic photomap for the mosquito Anopheles stephensi (Diptera : Culicidae): Application for physical mappingen
dc.title.serialJournal of Medical Entomologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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