Parasitic Plants Striga and Phelipanche Dependent upon Exogenous Strigolactones for Germination Have Retained Genes for Strigolactone Biosynthesis

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dc.contributor.authorDas, Malayen
dc.contributor.authorFernández-Aparicio, Mónicaen
dc.contributor.authorYang, Zhenzhenen
dc.contributor.authorHuang, Kanen
dc.contributor.authorWickett, Norman J.en
dc.contributor.authorAlford, Shannon R.en
dc.contributor.authorWafula, Eric K.en
dc.contributor.authordePamphilis, Claude W.en
dc.contributor.authorBouwmeester, Harroen
dc.contributor.authorTimko, Michael P.en
dc.contributor.authorYoder, John I.en
dc.contributor.authorWestwood, James H.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-10-02T17:27:36Zen
dc.date.available2019-10-02T17:27:36Zen
dc.date.issued2015-05en
dc.description.abstractStrigolactones are plant hormones with multiple functions, including regulating various aspects of plant architecture such as shoot branching, facilitating the colonization of plant roots by arbuscular mycorrhizal fungi, and acting as seed germination stimulants for certain parasitic plants of the family Orobanchaceae. The obligate parasitic species Phelipanche aegyptiaca and Striga hermonthica require strigolactones for germination, while the facultative parasite Triphysaria versicolor does not. It has been hypothesized that P. aegyptiaca and S. hermonthica would have undergone evolutionary loss of strigolactone biosynthesis as a part of their mechanism to enable specific detection of exogenous strigolactones. We analyzed the transcriptomes of P. aegyptiaca, S. hermonthica and T. versicolor and identified genes known to act in strigolactone synthesis (D27, CCD7, CCD8, and MAX1), perception (MAX2 and D14) and transport (PDR12). These genes were then analyzed to assess likelihood of function. Transcripts of all strigolactone-related genes were found in P. aegyptiaca and S. hermonthica, and evidence points to their encoding functional proteins. Gene open reading frames were consistent with homologs from Arabidopsis and other strigolactone-producing plants, and all genes were expressed in parasite tissues. In general, the genes related to strigolactone synthesis and perception appeared to be evolving under codon-based selective constraints in strigolactone-dependent species. Bioassays of S. hermonthica root extracts indicated the presence of strigolactone class stimulants on germination of P. aegyptiaca seeds. Taken together, these results indicate that Phelipanche aegyptiaca and S. hermonthica have retained functional genes involved in strigolactone biosynthesis, suggesting that the parasites use both endogenous and exogenous strigolactones and have mechanisms to differentiate the two.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.4236/ajps.2015.68120en
dc.identifier.issn2158-2742en
dc.identifier.urihttp://hdl.handle.net/10919/94323en
dc.identifier.volume6en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBroomrapeen
dc.subjectPhelipancheen
dc.subjectStrigaen
dc.subjectStrigolactoneen
dc.subjectTriphysariaen
dc.subjectWitchweeden
dc.titleParasitic Plants Striga and Phelipanche Dependent upon Exogenous Strigolactones for Germination Have Retained Genes for Strigolactone Biosynthesisen
dc.title.serialAmerican Journal of Plant Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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