Germline excision of transgenes in Aedes aegypti by homing endonucleases

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dc.contributor.authorAryan, Azadehen
dc.contributor.authorAnderson, Michelle A. E.en
dc.contributor.authorMyles, Kevin M.en
dc.contributor.authorAdelman, Zach N.en
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessed2014-06-13en
dc.date.accessioned2014-06-16T14:11:23Zen
dc.date.available2014-06-16T14:11:23Zen
dc.date.issued2013-04en
dc.description.abstractAedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.en
dc.description.sponsorshipNIH-NIAID AI071208en
dc.description.sponsorshipFralin Life Science Institute and College of Agriculture and Life Sciences at Virginia Techen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAryan, A.; Anderson, M. A. E.; Myles, K. M.; Adelman, Z. N., "Germline excision of transgenes in Aedes aegypti by homing endonucleases," Scientific Reports 3:1603, (2013). DOI: 10.1038/srep01603.en
dc.identifier.doihttps://doi.org/10.1038/srep01603en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://hdl.handle.net/10919/48929en
dc.identifier.urlhttp://www.nature.com/srep/2013/130403/srep01603/full/srep01603.htmlen
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectengineered male mosquitosen
dc.subjectyellow-fever mosquitoen
dc.subjecthomologousen
dc.subjectrecombinationen
dc.subjectdirected evolutionen
dc.subjectanopheles-gambiaeen
dc.subjectdna breaksen
dc.subjectgeneen
dc.subjectdrosophilaen
dc.subjectsequencesen
dc.subjectpopulationsen
dc.subjectmultidisciplinary sciencesen
dc.titleGermline excision of transgenes in Aedes aegypti by homing endonucleasesen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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