La Crosse Virus in Southwestern Virginia: Role of Exotic Mosquito Species and Effect of Virus Infection on Feeding

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dc.contributor.authorJackson, Bryan Tyleren
dc.contributor.committeechairPaulson, Sally L.en
dc.contributor.committeememberRoberts, E. Andersonen
dc.contributor.committeememberBrewster, Carlyle C.en
dc.contributor.committeememberYoungman, Roger R.en
dc.contributor.committeememberBelden, Lisa K.en
dc.contributor.departmentEntomologyen
dc.date.accessioned2014-03-14T20:12:59Zen
dc.date.adate2009-06-26en
dc.date.available2014-03-14T20:12:59Zen
dc.date.issued2009-05-29en
dc.date.rdate2013-01-02en
dc.date.sdate2009-06-12en
dc.description.abstractThe family Bunyaviridae is the largest of vertebrate diseases and includes the mosquito-borne disease La Crosse (LAC) virus. Vectors include the major vector Aedes triseriatus and two accessory vectors Aedes albopictus and Aedes japonicus. In the past several decades there has been an increase in the number of LAC cases, implication of new vectors, and the appearance of new foci of disease in the southeastern U.S. To better understand the vectors and the relationship between vectors and the virus, laboratory and field studies were conducted to determine oviposition preferences, effect of virus infection on blood-feeding behavior, and compare the efficacy of various methods to determine minimum infection rates of vectors. In laboratory studies of oviposition preference, only Ae. japonicus demonstrated a preference when presented with preexisting eggs. They deposited more eggs in cups containing either conspecifics or Ae. albopictus. The presence of 1st instar larvae Ae. albopictus larvae deterred oviposition by Ae. triseriatus and Ae. japonicus. Ae. japonicus and Ae. triseriatus preferred cups containing larval rearing water (LRW) of conspecifics and Ae. albopictus. Aedes albopictus preferred LRW regardless of species compared to control cups. Field experiments with fresh egg papers and preexisting eggs did not show significant differences, although the unequal population densities of species in the study area confounded the analysis. More work is needed to elucidate the interaction among these species and its effect on oviposition in the field. Blood-feeding experiments showed that LAC virus-infected Ae. triseriatus and Ae. albopictus imbibed significantly less blood compared to uninfected mosquitoes. Because blood meal size affects the subsequent inhibition of host seeking, experiments were done to ascertain the effect of virus infection on refeeding. Significantly more infected Ae. triseriatus mosquitoes refed but there was no effect on the refeeding rate of Ae. albopictus. Thus, the detrimental effect of virus infection, i.e., reduction in blood meal size, may lead to increased host exposure by Ae. triseriatus, enhancing horizontal transmission. Collecting adult mosquitoes was more efficient to detect virus in field populations than the collection of eggs. Maximum likelihood estimation-infection rates (MLE-IR) were calculated using bias-corrected maximum likelihood estimation. Adult collections yielded significantly more positive pools compared with egg collections. Virus was isolated from pools from Ae. canadensis, Ae. triseriatus, and Ae. albopictus. These results are comparable to other studies.en
dc.description.degreePh. D.en
dc.identifier.otheretd-06122009-113622en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06122009-113622/en
dc.identifier.urihttp://hdl.handle.net/10919/28029en
dc.publisherVirginia Techen
dc.relation.haspartJackson_ETD.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectBlood-feeding behavioren
dc.subjectInfection ratesen
dc.subjectOviposition preferencesen
dc.subjectLa Crosse virusen
dc.titleLa Crosse Virus in Southwestern Virginia: Role of Exotic Mosquito Species and Effect of Virus Infection on Feedingen
dc.typeDissertationen
thesis.degree.disciplineEntomologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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