Establishment and application of an accurate identification method for fragrant soybeans

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dc.contributor.authorZhang, Yong-fangen
dc.contributor.authorZhang, Chun-yanen
dc.contributor.authorZhang, Boen
dc.contributor.authorYin, Manen
dc.contributor.authorHong, Hui-longen
dc.contributor.authorYu, Li-lien
dc.contributor.authorGao, Hua-weien
dc.contributor.authorGu, Yong-zheen
dc.contributor.authorLiu, Zhang-xiongen
dc.contributor.authorLi, Fu-hengen
dc.contributor.authorQiu, Li-Juanen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-07-29T12:47:02Zen
dc.date.available2021-07-29T12:47:02Zen
dc.date.issued2021-05en
dc.description.abstractIn order to screen the aroma characteristics of soybean, a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline (2-AP), an important compound related to soybean aroma, using gas chromatography-mass spectrometry (GC-MS). Based on peak profile, total peak area and retention time as test indexes, an accurate identification method for fragrant soybeans was established. The optimum parameters of the protocol consisted of column temperature 70 degrees C, sample injector temperature 180 degrees C, optimum extraction alcohol content 1 mL, NaCl content 0.1 g, ultrasonication time 10 min, and extraction time 1 h, which were established by using the orthogonal test of single factors and three factors with four levels (L-9(3)(4)). 2-AP content of leaves had significant correlations with seeds, which were easier to measure. The protocol was simple and easy to carry out, consumed only small amounts of reagents, and provided accurate and reliable results with good reproducibility. A total of 101 soybean genotypes from different geographical sources were analyzed using this protocol. The results showed that the average content of 2-AP was 0.29 mg L-1, ranging from 0.094 to 1.816 mg L-1, and the genetic diversity index was 0.54. Among all genotypes-tested, they were classified into three grades, including seven elite genotypes identified as "grade one fragrant soybeans", which were Zhonglong 608, Heinong 88, Ha13-2958, Hongmiandou, Heinong 82, Huangmaodou, and Jiyu 21. These results provide both an identification technique and several elite aroma genotypes for gene discovery and good quality breeding in soybean.en
dc.description.notesThis work was supported by the National Key R&D Program for Crop Breeding (2016YFD0100201) and Scientific Innovation Program of of the Chinese Academy of Agricultural Sciences (CAAS) . We thank the Core Facility Platform, Institute of Crop Sciences, CAAS for their assistance with GC-MS analyses.en
dc.description.sponsorshipNational Key R&D Program for Crop Breeding [2016YFD0100201]; Scientific Innovation Program of of the Chinese Academy of Agricultural Sciences (CAAS)en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/S2095-3119(20)63328-7en
dc.identifier.issn2095-3119en
dc.identifier.issue5en
dc.identifier.urihttp://hdl.handle.net/10919/104437en
dc.identifier.volume20en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectsoybeanen
dc.subject2-acetyl-1pyrrolineen
dc.subjectGC-MSen
dc.subjectquantification methoden
dc.subjectgermplasmen
dc.titleEstablishment and application of an accurate identification method for fragrant soybeansen
dc.title.serialJournal of Integrative Agricultureen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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