Integrated proteomic and transcriptomic analysis of the Aedes aegypti eggshell

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dc.contributor.authorMarinotti, Osvaldoen
dc.contributor.authorNgo, Tuanen
dc.contributor.authorKojin, Bianca B.en
dc.contributor.authorChou, Shao-Peien
dc.contributor.authorNguyen, Brianen
dc.contributor.authorJuhn, Jenniferen
dc.contributor.authorCarballar-LejarazĂș, Rebecaen
dc.contributor.authorMarinotti, Pedro N.en
dc.contributor.authorJiang, Xiaofangen
dc.contributor.authorWalter, Marika F.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorGershon, Paul D.en
dc.contributor.authorJames, Anthony A.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2015-11-18T07:02:11Zen
dc.date.available2015-11-18T07:02:11Zen
dc.date.issued2014-04-05en
dc.date.updated2015-11-18T07:02:11Zen
dc.description.abstractBackground Mosquito eggshells show remarkable diversity in physical properties and structure consistent with adaptations to the wide variety of environments exploited by these insects. We applied proteomic, transcriptomic, and hybridization in situ techniques to identify gene products and pathways that participate in the assembly of the Aedes aegypti eggshell. Aedes aegypti population density is low during cold and dry seasons and increases immediately after rainfall. The survival of embryos through unfavorable periods is a key factor in the persistence of their populations. The work described here supports integrated vector control approaches that target eggshell formation and result in Ae. aegypti drought-intolerant phenotypes for public health initiatives directed to reduce mosquito-borne diseases. Results A total of 130 proteins were identified from the combined mass spectrometric analyses of eggshell preparations. Conclusions Classification of proteins according to their known and putative functions revealed the complexity of the eggshell structure. Three novel Ae. aegypti vitelline membrane proteins were discovered. Odorant-binding and cysteine-rich proteins that may be structural components of the eggshell were identified. Enzymes with peroxidase, laccase and phenoloxidase activities also were identified, and their likely involvements in cross-linking reactions that stabilize the eggshell structure are discussed.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Developmental Biology. 2014 Apr 05;14(1):15en
dc.identifier.doihttps://doi.org/10.1186/1471-213X-14-15en
dc.identifier.urihttp://hdl.handle.net/10919/64168en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderMarinotti et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIntegrated proteomic and transcriptomic analysis of the Aedes aegypti eggshellen
dc.title.serialBMC Developmental Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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