Improved reference genome of Aedes aegypti informs arbovirus vector control

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dc.contributor.authorMatthews, Benjamin J.en
dc.contributor.authorDudchenko, Olgaen
dc.contributor.authorKingan, Sarah B.en
dc.contributor.authorKoren, Sergeyen
dc.contributor.authorAntoshechkin, Igoren
dc.contributor.authorCrawford, Jacob E.en
dc.contributor.authorGlassford, William J.en
dc.contributor.authorHerre, Margareten
dc.contributor.authorRedmond, Seth N.en
dc.contributor.authorRose, Noah H.en
dc.contributor.authorWeedall, Gareth D.en
dc.contributor.authorWu, Yangen
dc.contributor.authorBatra, Sanjit S.en
dc.contributor.authorBrito-Sierra, Carlos A.en
dc.contributor.authorBuckingham, Steven D.en
dc.contributor.authorCampbell, Corey L.en
dc.contributor.authorChan, Sakien
dc.contributor.authorCox, Ericen
dc.contributor.authorEvans, Benjamin R.en
dc.contributor.authorFansiri, Thanyalaken
dc.contributor.authorFilipovic, Igoren
dc.contributor.authorFontaine, Albinen
dc.contributor.authorGloria-Soria, Andreaen
dc.contributor.authorHall, Richarden
dc.contributor.authorJoardar, Vinita S.en
dc.contributor.authorJones, Andrew K.en
dc.contributor.authorKay, Raissa G. G.en
dc.contributor.authorKodali, Vamsi K.en
dc.contributor.authorLee, Joyceen
dc.contributor.authorLycett, Gareth J.en
dc.contributor.authorMitchell, Sara N.en
dc.contributor.authorMuehling, Jillen
dc.contributor.authorMurphy, Michael R.en
dc.contributor.authorOmer, Arina D.en
dc.contributor.authorPartridge, Frederick A.en
dc.contributor.authorPeluso, Paulen
dc.contributor.authorAiden, Aviva Presseren
dc.contributor.authorRamasamy, Vidyaen
dc.contributor.authorRasic, Gordanaen
dc.contributor.authorRoy, Souraven
dc.contributor.authorSaavedra-Rodriguez, Karlaen
dc.contributor.authorSharan, Shrutien
dc.contributor.authorSharma, Atashien
dc.contributor.authorSmith, Melissa Lairden
dc.contributor.authorTurner, Joeen
dc.contributor.authorWeakley, Allison M.en
dc.contributor.authorZhao, Zhileien
dc.contributor.authorAkbari, Omar S.en
dc.contributor.authorBlack, William C.en
dc.contributor.authorCao, Hanen
dc.contributor.authorDarby, Alistair C.en
dc.contributor.authorHill, Catherine A.en
dc.contributor.authorJohnston, J. Spenceren
dc.contributor.authorMurphy, Terence D.en
dc.contributor.authorRaikhel, Alexander S.en
dc.contributor.authorSattelle, David B.en
dc.contributor.authorSharakhov, Igor V.en
dc.contributor.authorWhite, Bradley J.en
dc.contributor.authorZhao, Lien
dc.contributor.authorAiden, Erez Liebermanen
dc.contributor.authorMann, Richard S.en
dc.contributor.authorLambrechts, Louisen
dc.contributor.authorPowell, Jeffrey R.en
dc.contributor.authorSharakhova, Maria V.en
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorRobertson, Hugh M.en
dc.contributor.authorMcBride, Carolyn S.en
dc.contributor.authorHastic, Alex R.en
dc.contributor.authorKorlach, Jonasen
dc.contributor.authorNeafsey, Daniel E.en
dc.contributor.authorPhillippy, Adam M.en
dc.contributor.authorVosshall, Leslie B.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentEntomologyen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessioned2019-09-10T13:19:43Zen
dc.date.available2019-09-10T13:19:43Zen
dc.date.issued2018-11-22en
dc.description.abstractFemale Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector.en
dc.description.notesWe thank R. Andino; S. Emrich and D. Lawson (Vectorbase); A. A. James, M. Kunitomi, C. Nusbaum, D. Severson, N. Whiteman; T. Dickinson, M. Hartley and B. Rice (Dovetail Genomics) for early participation in the AGWG; C. Bargmann, D. Botstein, E. Jarvis and E. Lander for encouragement and facilitation. N. Keivanfar, D. Jaffe and D. M. Church (10X Genomics) prepared DNA for structural-variant analysis. We thank A. Harmon of the New York Times and acknowledge generous pro bono data and analysis from our corporate collaborators. This research was supported in part by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under grant number U19AI110818 to the Broad Institute (S.N.R. and D.E.N.); USDA 2017-05741 (E.L.A.); NSF PHY-1427654 Center for Theoretical Biological Physics (E.L.A.); NIH Intramural Research Program, National Library of Medicine and National Human Genome Research Institute (A.M.P. and S.K.) and the following extramural NIH grants: R01AI101112 (J.R.P.), R35GM118336 (R.S.M. and W.J.G.), R21AI121853 (M.V.S., I.V.S. and A. S.), R01AI123338 (Z.T.), T32GM007739 (M.H.), NIH/NCATS UL1TR000043 (Rockefeller University), DP2OD008540 (E.L.A.), U01AI088647, 1R01AI121211 (W.C.B. IV), Fogarty Training Grant D43TW001130-08, U01HL130010 (E.L.A.), UM1HG009375 (E.L.A), 5K22AI113060 (O.S.A.), 1R21AI123937 (O.S.A.), and R00DC012069 (C.S.M.); Defence Advanced Research Project Agency: HR0011-17-2-0047 (O.S.A.). Other support was provided by Jane Coffin Childs Memorial Fund (B.J.M.), Center for Theoretical Biological Physics postdoctoral fellowship (O.D.), Robertson Foundation (L.Z.), and McNair & Welch (Q-1866) Foundations (E.L.A.), French Government's Investissement d'Avenir program, Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID to L.L.), Agence Nationale de la Recherche grant ANR-17-ERC2-0016-01 (L.L.), European Union's Horizon 2020 research and innovation program under ZikaPLAN grant agreement no. 734584 (L.L.), Pew and Searle Scholars Programs (C.S.M.), Klingenstein-Simons Fellowship in the Neurosciences (C.S.M.). A.M.W., B.J.W., J.E.C. and S.N.M. were supported by Verily Life Sciences. L.B.V. is an investigator of the Howard Hughes Medical Institute.en
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [U19AI110818]; USDA [2017-05741]; NIH Intramural Research Program; National Library of Medicine; National Human Genome Research Institute; NSF [PHY-1427654]; NIH [R01AI101112, R35GM118336, R21AI121853, R01AI123338, T32GM007739, NIH/NCATS UL1TR000043, DP2OD008540, U01AI088647, 1R01AI121211, D43TW001130-08, U01HL130010, UM1HG009375, 5K22AI113060, 1R21AI123937, R00DC012069]; Defence Advanced Research Project Agency [HR0011-17-2-0047]; Jane Coffin Childs Memorial Fund; Center for Theoretical Biological Physics postdoctoral fellowship; Robertson Foundation; McNair Foundation; Welch Foundation [Q-1866]; French Government's Investissement d'Avenir program, Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases [ANR-10-LABX-62-IBEID]; Agence Nationale de la Recherche [ANR-17-ERC2-0016-01]; European Union [734584]; Pew and Searle Scholars Programs; Klingenstein-Simons Fellowship in the Neurosciences; Verily Life Sciencesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41586-018-0692-zen
dc.identifier.eissn1476-4687en
dc.identifier.issn0028-0836en
dc.identifier.issue7732en
dc.identifier.pmid30429615en
dc.identifier.urihttp://hdl.handle.net/10919/93520en
dc.identifier.volume563en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleImproved reference genome of Aedes aegypti informs arbovirus vector controlen
dc.title.serialNatureen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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