An Engineered Reporter Phage for the Fluorometric Detection of Escherichia coli in Ground Beef
| dc.contributor.author | Chen, Anqi | en |
| dc.contributor.author | Wang, Danhui | en |
| dc.contributor.author | Nugen, Sam R. | en |
| dc.contributor.author | Chen, Juhong | en |
| dc.contributor.department | Biological Systems Engineering | en |
| dc.date.accessioned | 2021-02-26T19:57:19Z | en |
| dc.date.available | 2021-02-26T19:57:19Z | en |
| dc.date.issued | 2021-02-19 | en |
| dc.date.updated | 2021-02-26T14:44:37Z | en |
| dc.description.abstract | Despite enhanced sanitation implementations, foodborne bacterial pathogens still remain a major threat to public health and generate high costs for the food industry. Reporter bacteriophage (phage) systems have been regarded as a powerful technology for diagnostic assays for their extraordinary specificity to target cells and cost-effectiveness. Our study introduced an enzyme-based fluorescent assay for detecting the presence of <i>E. coli</i> using the T7 phage engineered with the <i>lacZ</i> operon which encodes beta-galactosidase (β-gal). Both endogenous and overexpressed β-gal expression was monitored using a fluorescent-based method with 4-methylumbelliferyl β-<span style="font-variant: small-caps;">d</span>-galactopyranoside (MUG) as the substrate. The infection of <i>E. coli</i> with engineered phages resulted in a detection limit of 10 CFU/mL in ground beef juice after 7 h of incubation. In this study, we demonstrated that the overexpression of β-gal coupled with a fluorogenic substrate can provide a straightforward and sensitive approach to detect the potential biological contamination in food samples. The results also suggested that this system can be applied to detect <i>E. coli</i> strains isolated from environmental samples, indicating a broader range of bacterial detection. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Chen, A.; Wang, D.; Nugen, S.R.; Chen, J. An Engineered Reporter Phage for the Fluorometric Detection of Escherichia coli in Ground Beef. Microorganisms 2021, 9, 436. | en |
| dc.identifier.doi | https://doi.org/10.3390/microorganisms9020436 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/102457 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | bacteria detection | en |
| dc.subject | engineered bacteriophages | en |
| dc.subject | fluorescent assay | en |
| dc.subject | beta-galactosidase | en |
| dc.subject | ground beef | en |
| dc.title | An Engineered Reporter Phage for the Fluorometric Detection of Escherichia coli in Ground Beef | en |
| dc.title.serial | Microorganisms | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |
| dc.type.dcmitype | StillImage | en |