A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure

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dc.contributor.authorBracey, Daniel N.en
dc.contributor.authorSeyler, Thorsten M.en
dc.contributor.authorJinnah, Alexander H.en
dc.contributor.authorLively, Mark O.en
dc.contributor.authorWilley, Jeffrey S.en
dc.contributor.authorSmith, Thomas L.en
dc.contributor.authorVan Dyke, Mark E.en
dc.contributor.authorWhitlock, Patrick W.en
dc.contributor.departmentBiomedical Engineering and Mechanicsen
dc.date.accessioned2018-09-21T16:41:49Zen
dc.date.available2018-09-21T16:41:49Zen
dc.date.issued2018-07-12en
dc.date.updated2018-09-21T07:13:11Zen
dc.description.abstractBackground: Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less commonly, xenografts which are taken from animal species. Xenografts are available in unlimited supply from healthy animal donors with controlled biology, avoiding the risk of human disease transmission, and may satisfy current demand for bone graft products. Methods: In the current study, cancellous bone was harvested from porcine femurs and subjected to a novel decellularization protocol to derive a bone scaffold. Results: The scaffold was devoid of donor cellular material on histology and DNA sampling (<i>p</i> &lt; 0.01). Microarchitectural properties important for osteoconductive potential were preserved after decellularization as shown by high resolution imaging modalities. Proteomics data demonstrated similar profiles when comparing the porcine bone scaffold against commercially available human demineralized bone matrix approved for clinical use. Conclusion: We are unaware of any porcine-derived bone graft products currently used in orthopaedic surgery practice. Results from the current study suggest that porcine-derived bone scaffolds warrant further consideration to serve as a potential bone graft substitute.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBracey, D.N.; Seyler, T.M.; Jinnah, A.H.; Lively, M.O.; Willey, J.S.; Smith, T.L.; Van Dyke, M.E.; Whitlock, P.W. A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure. J. Funct. Biomater. 2018, 9, 45.en
dc.identifier.doihttps://doi.org/10.3390/jfb9030045en
dc.identifier.urihttp://hdl.handle.net/10919/85072en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectxenograften
dc.subjectscaffolden
dc.subjectdecellularizeden
dc.subjectosteoconductiveen
dc.subjectbone graften
dc.subjectporcineen
dc.titleA Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structureen
dc.title.serialJournal of Functional Biomaterialsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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