Characterization and silencing of the fatty acid- and retinol-binding Pp-far-1 gene in Pratylenchus penetrans

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dc.contributor.authorVieira, Pauloen
dc.contributor.authorKamo, K.en
dc.contributor.authorEisenback, Jonathan D.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-03-19T17:36:16Zen
dc.date.available2020-03-19T17:36:16Zen
dc.date.issued2017-09en
dc.description.abstractFatty acid-and retinol-binding proteins (FARs) are unique to nematodes, and are implicated in a wide range of metabolic and parasitic related functions. Along with the in silico analyses performed in this study, three different FAR members of this family were identified in Pratylenchus penetrans. The cDNA corresponding to the fatty acid-and retinol-binding Pp-far-1 gene was cloned and herein characterized at the molecular level for the first time for this genus. The translated 185 amino acid sequence of P. penetrans FAR-1 sequence, with a predicted molecular weight of 20.82 kDa and a pI of 5.49, shares highest sequence identity to FARs of other migratory nematodes of the Pratylenchidae family (90% to P. vulnus and 80% to Radopholus similis). In situ hybridization localizes Pp-far-1 transcripts in the hypodermis of the nematode. RT-qPCR detected Pp-far-1 transcripts for all nematode developmental stages, with highest expression levels found in juveniles, adult females and adult males, respectively. Pp-far-1 is also highly expressed during infection and establishment of P. penetrans in roots of different host plants, such as corn, lily and soybean. The importance of Pp-far-1 was studied by in planta RNA interference (RNAi) assays using stable soybean hairy root lines. Targeting Pp-far-1 decreased expression of the nematode Pp-far-1 and significantly reduced the reproduction of nematodes, with a total of between 44% and 70% fewer nematodes in comparison to the average number of nematodes counted for control lines. The results indicate that suppressing the expression levels of Pp-far-1 can act as an effective target gene to control P. penetrans.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThe authors acknowledge the technical assistance of Sarah Shih. This material is based upon work that is supported by the National Institute of Food and Agriculture, US Department of Agriculture, under award number 11588909. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.en
dc.description.sponsorshipNational Institute of Food and Agriculture, US Department of AgricultureUnited States Department of Agriculture (USDA) [11588909]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/ppa.12664en
dc.identifier.eissn1365-3059en
dc.identifier.issn0032-0862en
dc.identifier.issue7en
dc.identifier.urihttp://hdl.handle.net/10919/97379en
dc.identifier.volume66en
dc.language.isoenen
dc.rightsCC0 1.0 Universalen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.subjectgene silencingen
dc.subjectLilium longiflorumen
dc.subjectPratylenchidaeen
dc.subjectRNAien
dc.subjectsoybean hairy rootsen
dc.subjectZea maysen
dc.titleCharacterization and silencing of the fatty acid- and retinol-binding Pp-far-1 gene in Pratylenchus penetransen
dc.title.serialPlant Pathologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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