Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study

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dc.contributor.authorRuger, Lauren N.en
dc.contributor.authorYang, Esteren
dc.contributor.authorCoutermarsh-Ott, Sherylen
dc.contributor.authorVickers, Ellianaen
dc.contributor.authorGannon, Jessicaen
dc.contributor.authorNightengale, Marlieen
dc.contributor.authorHsueh, Andyen
dc.contributor.authorCiepluch, Brittanyen
dc.contributor.authorDervisis, Nikolaosen
dc.contributor.authorVlaisavljevich, Elien
dc.contributor.authorKlahn, Shawnaen
dc.date.accessioned2023-06-20T19:37:00Zen
dc.date.available2023-06-20T19:37:00Zen
dc.date.issued2023en
dc.description.abstractPurpose Feline soft tissue sarcoma (STS) and injection site sarcoma (fISS) are rapidly growing tumors with low metastatic potential, but locally aggressive behavior. Histotripsy is a non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tissue. In this study, we investigated the in vivo safety and feasibility of histotripsy to treat fISS using a custom 1 MHz transducer. Materials and Methods Three cats with naturally-occurring STS were treated with histotripsy before surgical removal of the tumor 3 to 6 days later. Gross and histological analyses were used to characterize the ablation efficacy of the treatment, and routine immunohistochemistry and batched cytokine analysis were used to investigate the acute immunological effects of histotripsy. Results Results showed that histotripsy ablation was achievable and well-tolerated in all three cats. Precise cavitation bubble clouds were generated in all patients, and hematoxylin & eosin stained tissues revealed ablative damage in targeted regions. Immunohistochemical results identified an increase in IBA-1 positive cells in treated tissues, and no significant changes in cytokine concentrations were identified post-treatment. Conclusions Overall, the results of this study demonstrate the safety and feasibility of histotripsy to target and ablate superficial feline STS and fISS tumors and guide the clinical development of histotripsy devices for this application.en
dc.description.notesThis work was funded by the Focused Ultrasound Foundation, grant #453137. Author Lauren Ruger was supported by the Virginia Tech Institute for Critical Technology and Applied Science (ICTAS) Doctoral Scholars program throughout the duration of this work. Primary investigator Shawna Klahn is an iTHRIV Scholar. The iTHRIV Scholars Program is supported in part by the National Center for Advancing Translational Sciences of the National Institutes of Health under Award Numbers UL1TR003015 and KL2TR003016. This work was completed in memory of Belle, Lauren Ruger's beloved pet cat, who passed away of cancer in 2021 and continues to inspire Lauren's veterinary research efforts.en
dc.description.sponsorshipFocused Ultrasound Foundation [453137]; Virginia Tech Institute for Critical Technology and Applied Science (ICTAS) Doctoral Scholars program; National Center for Advancing Translational Sciences of the National Institutes of Health [UL1TR003015, KL2TR003016]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1080/02656736.2023.2210272en
dc.identifier.eissn1464-5157en
dc.identifier.issn0265-6736en
dc.identifier.issue1en
dc.identifier.other2210272en
dc.identifier.pmid37196996en
dc.identifier.urihttp://hdl.handle.net/10919/115462en
dc.identifier.volume40en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSoft tissue sarcomaen
dc.subjectfelineen
dc.subjecthistotripsyen
dc.subjectfocused ultrasounden
dc.subjectcanceren
dc.subjectablationen
dc.subjectimmuno-oncologyen
dc.titleHistotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility studyen
dc.title.serialInternational Journal of Hyperthermiaen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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